Regulation mechanism of autophagy-related protein LC3 by c-Jun in methotrexate resistant human choriocarcinoma JEG-3 cells

To explore the regulation mechanism of autophagy- related protein, microtubule- associated protein 1 light chain 3 (LC3), via c-Jun in methotrexate resistant human choriocarcinoma JEG-3 cell lines. Human choriocarcinoma JEG-3 cell lines, and methotrexate resistant choriocarcinoma JEG-3 (JEG-3/MTXR)...

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Veröffentlicht in:Chung-hua fu chʿan kʿo tsa chih 2014-07, Vol.49 (7), p.531
Hauptverfasser: Shen, Yun, Xiang, Yang, Xiao, Changji, Zhao, Jing
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Sprache:chi
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Zusammenfassung:To explore the regulation mechanism of autophagy- related protein, microtubule- associated protein 1 light chain 3 (LC3), via c-Jun in methotrexate resistant human choriocarcinoma JEG-3 cell lines. Human choriocarcinoma JEG-3 cell lines, and methotrexate resistant choriocarcinoma JEG-3 (JEG-3/MTXR) cell lines were used in our present study. Phosphorylation c-Jun (p-c-Jun) was evaluated after exposure to 0.02 ng/ml methotrexate for 72 hours in both cells by western blot. c-Jun gene was knockdown by small interference RNA (siRNA) in JEG-3/MTXR cells, and LC3 was evaluated by western blot and reverse transcription-PCR. The binding of LC3 promoter with c- Jun protein was detected via chromatin immunoprecipitation assay (ChIP) with or without 0.02 ng/ml methotrexate exposure. The results showed that p-c-Jun was up-regulated after methotrexate treatment for 72 hours (1.99 ± 0.20, versus 0.20 ± 0.06 at 0 hour; P < 0.05) by western blot analysis in JEG-3/MTXR cell lines. Further investigation demonstrated that c-Jun-
ISSN:0529-567X