LL37 induces VEGF expression in dental pulp cells through ERK signalling

Aim To examine the in vitro effects of LL37 on the expression of vascular endothelial growth factor (VEGF) in human pulp cells and to identify the intracellular signalling pathway involved. Methodology Pulp cells at passage 6 were treated with 10 μg mL−1 synthesized LL37, and an inhibition assay was performed with MAPK or NF‐κB inhibitors to determine the possible signalling pathway. VEGF mRNA, VEGF protein and phosphorylated ERK1/2 levels were determined by real‐time PCR, ELISA and Western blot, respectively. Data were analysed using t‐tests. Results LL37 significantly increased both the mRNA and protein levels of VEGF in pulp cells (P