In vivo absolute quantification for mouse muscle metabolites using an inductively coupled synthetic signal injection method and newly developed (1) H/(31) P dual tuned probe

To obtain robust estimates of (31) P metabolite content in mouse skeletal muscles using our recently developed MR absolute quantification method and a custom-built (1) H/(31) P dual tuned radiofrequency (RF) coil optimized for mouse leg. We designed and fabricated a probe consisting of two dual tuned (1) H/(31) P solenoid coils: one leg was inserted to each solenoid. The mouse leg volume coil was incorporated with injector coils for MR absolute quantification. The absolute quantification method uses a synthetic reference signal injection approach and solves several challenges in MR absolute quantification including changes of coil loading and receiver gains. The (1) H/(31) P dual tuned probe was composed of two separate solenoid coils, one for each leg, to increase coil filling factors and signal-to-noise ratio. Each solenoid was equipped with a second coil to allow injection of reference signals. (31) P metabolite concentrations determined for normal mice were well within the expected range reported in the literature. We developed an RF probe and an absolute quantification approach adapted for mouse skeletal muscle.