Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor
Cancer cells can express specific biomarkers, such as cell membrane proteins and signaling factors. Thus, finding biomarkers and delivering diagnostic agents are important in the diagnosis of cancer. In this study, we investigated a biomarker imaging agent for the diagnosis of hepatic cancers. The a...
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| Veröffentlicht in: | Nanotechnology 2013-11, Vol.24 (47), p.475103-475103 |
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| creator | Yang, Seung-Hyun Heo, Dan Lee, Eugene Kim, Eunjung Lim, Eun-Kyung Lee, Young Han Haam, Seungjoo Suh, Jin-Suck Huh, Yong-Min Yang, Jaemoon Park, Sahng Wook |
| description | Cancer cells can express specific biomarkers, such as cell membrane proteins and signaling factors. Thus, finding biomarkers and delivering diagnostic agents are important in the diagnosis of cancer. In this study, we investigated a biomarker imaging agent for the diagnosis of hepatic cancers. The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent bearing a galactosyl group was prepared using manganese ferrite nanoparticles (MFNP) and galactosylgluconic acid. The utility of the ASGPr-targetable imaging agent, galactosylated MFNP (G-MFNP) was assessed by several methods in ASGPr-expressing HepG2 cells as target cells and ASGPr-deficient MCF7 cells. Physical and chemical properties of G-MFNP were examined using Fourier-transform infrared spectroscopy, dynamic light scattering, zeta potential analysis, and transmission electron microscopy. No significant cytotoxicity was observed in either cell line. Targeting ability was assessed using flow cytometry, magnetic resonance imaging, inductively coupled plasma atomic emission spectroscopy, absorbance analysis, dark-field microscopy, Prussian blue staining, and transmission electron microscopy. We demonstrated that G-MFNP target successfully and bind to ASGPr-expressing HepG2 cells specifically. We suggest that these results will be useful in strategies for cancer diagnoses based on magnetic resonance imaging. |
| doi_str_mv | 10.1088/0957-4484/24/47/475103 |
| format | Article |
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Thus, finding biomarkers and delivering diagnostic agents are important in the diagnosis of cancer. In this study, we investigated a biomarker imaging agent for the diagnosis of hepatic cancers. The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent bearing a galactosyl group was prepared using manganese ferrite nanoparticles (MFNP) and galactosylgluconic acid. The utility of the ASGPr-targetable imaging agent, galactosylated MFNP (G-MFNP) was assessed by several methods in ASGPr-expressing HepG2 cells as target cells and ASGPr-deficient MCF7 cells. Physical and chemical properties of G-MFNP were examined using Fourier-transform infrared spectroscopy, dynamic light scattering, zeta potential analysis, and transmission electron microscopy. No significant cytotoxicity was observed in either cell line. Targeting ability was assessed using flow cytometry, magnetic resonance imaging, inductively coupled plasma atomic emission spectroscopy, absorbance analysis, dark-field microscopy, Prussian blue staining, and transmission electron microscopy. We demonstrated that G-MFNP target successfully and bind to ASGPr-expressing HepG2 cells specifically. We suggest that these results will be useful in strategies for cancer diagnoses based on magnetic resonance imaging.</description><identifier>ISSN: 0957-4484</identifier><identifier>EISSN: 1361-6528</identifier><identifier>DOI: 10.1088/0957-4484/24/47/475103</identifier><identifier>PMID: 24192299</identifier><identifier>CODEN: NNOTER</identifier><language>eng</language><publisher>Bristol: IOP Publishing</publisher><subject>Absorbance ; Asialoglycoprotein Receptor - metabolism ; Colloids - chemistry ; Cross-disciplinary physics: materials science; rheology ; Electron microscopy ; Exact sciences and technology ; Ferric Compounds - metabolism ; Flow cytometry ; Fluorescence ; Galactose - metabolism ; Glycosylation ; Hep G2 Cells ; Humans ; Imaging ; Inductively coupled plasma ; Magnetic Resonance Imaging ; Manganese Compounds - metabolism ; Materials science ; MCF-7 Cells ; Microscopy ; Nanocrystalline materials ; Nanoparticles - chemistry ; Nanoparticles - ultrastructure ; Nanoscale materials and structures: fabrication and characterization ; Particle Size ; Physics ; Spectrophotometry, Atomic ; Spectroscopy, Fourier Transform Infrared ; Staining ; Static Electricity</subject><ispartof>Nanotechnology, 2013-11, Vol.24 (47), p.475103-475103</ispartof><rights>2013 IOP Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-99f9619277d2f9a5d846fd6e645fd70c079b61657c2bf8294dc1fca082bc3b683</citedby><cites>FETCH-LOGICAL-c417t-99f9619277d2f9a5d846fd6e645fd70c079b61657c2bf8294dc1fca082bc3b683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/0957-4484/24/47/475103/pdf$$EPDF$$P50$$Giop$$H</linktopdf><link.rule.ids>314,780,784,27924,27925,53846,53893</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27967990$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24192299$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Seung-Hyun</creatorcontrib><creatorcontrib>Heo, Dan</creatorcontrib><creatorcontrib>Lee, Eugene</creatorcontrib><creatorcontrib>Kim, Eunjung</creatorcontrib><creatorcontrib>Lim, Eun-Kyung</creatorcontrib><creatorcontrib>Lee, Young Han</creatorcontrib><creatorcontrib>Haam, Seungjoo</creatorcontrib><creatorcontrib>Suh, Jin-Suck</creatorcontrib><creatorcontrib>Huh, Yong-Min</creatorcontrib><creatorcontrib>Yang, Jaemoon</creatorcontrib><creatorcontrib>Park, Sahng Wook</creatorcontrib><title>Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor</title><title>Nanotechnology</title><addtitle>Nano</addtitle><addtitle>Nanotechnology</addtitle><description>Cancer cells can express specific biomarkers, such as cell membrane proteins and signaling factors. Thus, finding biomarkers and delivering diagnostic agents are important in the diagnosis of cancer. In this study, we investigated a biomarker imaging agent for the diagnosis of hepatic cancers. The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent bearing a galactosyl group was prepared using manganese ferrite nanoparticles (MFNP) and galactosylgluconic acid. The utility of the ASGPr-targetable imaging agent, galactosylated MFNP (G-MFNP) was assessed by several methods in ASGPr-expressing HepG2 cells as target cells and ASGPr-deficient MCF7 cells. Physical and chemical properties of G-MFNP were examined using Fourier-transform infrared spectroscopy, dynamic light scattering, zeta potential analysis, and transmission electron microscopy. No significant cytotoxicity was observed in either cell line. Targeting ability was assessed using flow cytometry, magnetic resonance imaging, inductively coupled plasma atomic emission spectroscopy, absorbance analysis, dark-field microscopy, Prussian blue staining, and transmission electron microscopy. We demonstrated that G-MFNP target successfully and bind to ASGPr-expressing HepG2 cells specifically. We suggest that these results will be useful in strategies for cancer diagnoses based on magnetic resonance imaging.</description><subject>Absorbance</subject><subject>Asialoglycoprotein Receptor - metabolism</subject><subject>Colloids - chemistry</subject><subject>Cross-disciplinary physics: materials science; rheology</subject><subject>Electron microscopy</subject><subject>Exact sciences and technology</subject><subject>Ferric Compounds - metabolism</subject><subject>Flow cytometry</subject><subject>Fluorescence</subject><subject>Galactose - metabolism</subject><subject>Glycosylation</subject><subject>Hep G2 Cells</subject><subject>Humans</subject><subject>Imaging</subject><subject>Inductively coupled plasma</subject><subject>Magnetic Resonance Imaging</subject><subject>Manganese Compounds - metabolism</subject><subject>Materials science</subject><subject>MCF-7 Cells</subject><subject>Microscopy</subject><subject>Nanocrystalline materials</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - ultrastructure</subject><subject>Nanoscale materials and structures: fabrication and characterization</subject><subject>Particle Size</subject><subject>Physics</subject><subject>Spectrophotometry, Atomic</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Staining</subject><subject>Static Electricity</subject><issn>0957-4484</issn><issn>1361-6528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVpaLZp_0LQpdCLu5Is6-NYQpMUUgolPYuxLBkFreVK2sP--2qz2_SYYWDm8MzXOwhdU_KFEqW2RA-y41zxLeNbLpsPlPRv0Ib2gnZiYOot2rxAl-h9KU-EUKoYfYcuGaeaMa03yN1BBFtTOUSobsI7WGZYXHHYu5xDdXiBJa2Qa7DRFexTxhXy7I7wj1847GAOy4yTx1ACxDTHg01rTtWFBWdn3VpT_oAuPMTiPp7jFfp9--3x5r57-Hn3_ebrQ2c5lbXT2mvRNpNyYl7DMCku_CSc4IOfJLFE6lFQMUjLRq-Y5pOl3gJRbLT9KFR_hT6f-rYF_uxdqWYXinUxtpPSvhg6MKJ71ex1lGsiFe173lBxQm1OpWTnzZrb3flgKDHHb5ij0OYotGHc8JY-f6MVXp9n7Medm17K_snfgE9nAIqF6DMsNpT_nNRCak0ax05cSKt5Svu8NBVfm_4XgKmiRA</recordid><startdate>20131129</startdate><enddate>20131129</enddate><creator>Yang, Seung-Hyun</creator><creator>Heo, Dan</creator><creator>Lee, Eugene</creator><creator>Kim, Eunjung</creator><creator>Lim, Eun-Kyung</creator><creator>Lee, Young Han</creator><creator>Haam, Seungjoo</creator><creator>Suh, Jin-Suck</creator><creator>Huh, Yong-Min</creator><creator>Yang, Jaemoon</creator><creator>Park, Sahng Wook</creator><general>IOP Publishing</general><general>Institute of Physics</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20131129</creationdate><title>Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor</title><author>Yang, Seung-Hyun ; Heo, Dan ; Lee, Eugene ; Kim, Eunjung ; Lim, Eun-Kyung ; Lee, Young Han ; Haam, Seungjoo ; Suh, Jin-Suck ; Huh, Yong-Min ; Yang, Jaemoon ; Park, Sahng Wook</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-99f9619277d2f9a5d846fd6e645fd70c079b61657c2bf8294dc1fca082bc3b683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Absorbance</topic><topic>Asialoglycoprotein Receptor - metabolism</topic><topic>Colloids - chemistry</topic><topic>Cross-disciplinary physics: materials science; rheology</topic><topic>Electron microscopy</topic><topic>Exact sciences and technology</topic><topic>Ferric Compounds - metabolism</topic><topic>Flow cytometry</topic><topic>Fluorescence</topic><topic>Galactose - metabolism</topic><topic>Glycosylation</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>Imaging</topic><topic>Inductively coupled plasma</topic><topic>Magnetic Resonance Imaging</topic><topic>Manganese Compounds - metabolism</topic><topic>Materials science</topic><topic>MCF-7 Cells</topic><topic>Microscopy</topic><topic>Nanocrystalline materials</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - ultrastructure</topic><topic>Nanoscale materials and structures: fabrication and characterization</topic><topic>Particle Size</topic><topic>Physics</topic><topic>Spectrophotometry, Atomic</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Staining</topic><topic>Static Electricity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Seung-Hyun</creatorcontrib><creatorcontrib>Heo, Dan</creatorcontrib><creatorcontrib>Lee, Eugene</creatorcontrib><creatorcontrib>Kim, Eunjung</creatorcontrib><creatorcontrib>Lim, Eun-Kyung</creatorcontrib><creatorcontrib>Lee, Young Han</creatorcontrib><creatorcontrib>Haam, Seungjoo</creatorcontrib><creatorcontrib>Suh, Jin-Suck</creatorcontrib><creatorcontrib>Huh, Yong-Min</creatorcontrib><creatorcontrib>Yang, Jaemoon</creatorcontrib><creatorcontrib>Park, Sahng Wook</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Nanotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Seung-Hyun</au><au>Heo, Dan</au><au>Lee, Eugene</au><au>Kim, Eunjung</au><au>Lim, Eun-Kyung</au><au>Lee, Young Han</au><au>Haam, Seungjoo</au><au>Suh, Jin-Suck</au><au>Huh, Yong-Min</au><au>Yang, Jaemoon</au><au>Park, Sahng Wook</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor</atitle><jtitle>Nanotechnology</jtitle><stitle>Nano</stitle><addtitle>Nanotechnology</addtitle><date>2013-11-29</date><risdate>2013</risdate><volume>24</volume><issue>47</issue><spage>475103</spage><epage>475103</epage><pages>475103-475103</pages><issn>0957-4484</issn><eissn>1361-6528</eissn><coden>NNOTER</coden><abstract>Cancer cells can express specific biomarkers, such as cell membrane proteins and signaling factors. Thus, finding biomarkers and delivering diagnostic agents are important in the diagnosis of cancer. In this study, we investigated a biomarker imaging agent for the diagnosis of hepatic cancers. The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent bearing a galactosyl group was prepared using manganese ferrite nanoparticles (MFNP) and galactosylgluconic acid. The utility of the ASGPr-targetable imaging agent, galactosylated MFNP (G-MFNP) was assessed by several methods in ASGPr-expressing HepG2 cells as target cells and ASGPr-deficient MCF7 cells. Physical and chemical properties of G-MFNP were examined using Fourier-transform infrared spectroscopy, dynamic light scattering, zeta potential analysis, and transmission electron microscopy. No significant cytotoxicity was observed in either cell line. Targeting ability was assessed using flow cytometry, magnetic resonance imaging, inductively coupled plasma atomic emission spectroscopy, absorbance analysis, dark-field microscopy, Prussian blue staining, and transmission electron microscopy. We demonstrated that G-MFNP target successfully and bind to ASGPr-expressing HepG2 cells specifically. We suggest that these results will be useful in strategies for cancer diagnoses based on magnetic resonance imaging.</abstract><cop>Bristol</cop><pub>IOP Publishing</pub><pmid>24192299</pmid><doi>10.1088/0957-4484/24/47/475103</doi><tpages>9</tpages></addata></record> |
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| subjects | Absorbance Asialoglycoprotein Receptor - metabolism Colloids - chemistry Cross-disciplinary physics: materials science rheology Electron microscopy Exact sciences and technology Ferric Compounds - metabolism Flow cytometry Fluorescence Galactose - metabolism Glycosylation Hep G2 Cells Humans Imaging Inductively coupled plasma Magnetic Resonance Imaging Manganese Compounds - metabolism Materials science MCF-7 Cells Microscopy Nanocrystalline materials Nanoparticles - chemistry Nanoparticles - ultrastructure Nanoscale materials and structures: fabrication and characterization Particle Size Physics Spectrophotometry, Atomic Spectroscopy, Fourier Transform Infrared Staining Static Electricity |
| title | Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor |
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