Analysis of acyclovir in vitreous humor by a validated HPLC method

An HPLC-UV method was developed and validated for the determination of acyclovir in vitreous humor. The method was carried out in isocratic mode using 0.02 mol/L acetic acid/methanol (95:5) as mobile phase, a C18 column at 25 °C and UV detection at 254 nm. The method was linear (r2 > 0.99) over t...

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Veröffentlicht in:	Pharmazie 2013-04, Vol.68 (4), p.235-239
Hauptverfasser:	Silva, G R, Caldeira, A S P, Damico, F M, Takahashi, B S, Silva-Cunha, A, Fialho, S L
Format:	Artikel
Sprache:	eng
Schlagworte:	Acyclovir - analysis Animals Antiviral Agents - analysis Chromatography, High Pressure Liquid Indicators and Reagents Limit of Detection Rabbits Reference Standards Reproducibility of Results Retinal Necrosis Syndrome, Acute - drug therapy Solutions Spectrophotometry, Ultraviolet Vitreous Body - chemistry
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Zusammenfassung:	An HPLC-UV method was developed and validated for the determination of acyclovir in vitreous humor. The method was carried out in isocratic mode using 0.02 mol/L acetic acid/methanol (95:5) as mobile phase, a C18 column at 25 °C and UV detection at 254 nm. The method was linear (r2 > 0.99) over the range of 35-700 μg/mL, precise (RSD < 5%), accurate (recovery ranged from 98.18 to 99.64%), robust, selective regarding of the vitreous humor, and robust remaining unaffected by deliberate variations in relevant parameters. The validated HPLC-UV method can be successfully applied to determine acyclovir directly injected into the vitreous cavity of rabbits' eye.
ISSN:	0031-7144
DOI:	10.1691/ph.2013.2153