Isolation and Gene Expression Analysis of a Papain-Type Cysteine Protease in Thermogenic Skunk Cabbage (Symplocarpus renifolius)

Skunk cabbage (Symplocarpus renifolius) spadices contain abundant transcripts for cysteine protease (CP). From thermogenic spadices, we isolated SrCPA, a highly expressed CP gene that encoded a papain-type CP. SrCPA is structurally similar to other plant CPs, including the senescence-associated CPs...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2012, Vol.76 (10), p.1990-1992
Hauptverfasser:	ITO-INABA, Yasuko, MASUKO, Hiromi, WATANABE, Masao, INABA, Takehito
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Araceae - enzymology Araceae - genetics Araceae - growth & development Base Sequence Biological and medical sciences Brassica Cloning, Molecular CP gene cysteine protease DNA, Complementary - genetics floral development Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant Molecular Sequence Data Papain - chemistry Papain - genetics RNA, Messenger - genetics RNA, Messenger - metabolism Symplocarpus renifolius thermogenesis
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Zusammenfassung:	Skunk cabbage (Symplocarpus renifolius) spadices contain abundant transcripts for cysteine protease (CP). From thermogenic spadices, we isolated SrCPA, a highly expressed CP gene that encoded a papain-type CP. SrCPA is structurally similar to other plant CPs, including the senescence-associated CPs found in aroids. The expression of SrCPA increased during floral development, and was observed in all floral tissues except for the stamens.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.120434