Dynamics of lymphocyte subsets in children living in an area polluted by polychlorinated biphenyls

Immune system development, particularly in the pre-natal and early post-natal periods, has far-reaching health consequences during childhood, as well as throughout life. Exposure to poly-chlorinated biphenyls (PCBs) during pre-natal and early life has been previously associated with changes in the incidence of infectious and allergic diseases in children, and humoral immunity alterations. Lymphocyte immunophenotyping is an important tool in the diagnosis of immunologic and hematologic disorders. This study used a lysed whole blood method for analysis of lymphocyte sub-populations in samples from children born and living in two districts: a highly-contaminated area (Michalovce) and one (Svidnik/Stropkov) with 2-fold lower environmental PCB levels. The percentages of B-lymphocytes (CD19+), activated HLADR+CD19+ cells, and CD8+ T-lymphocytes significantly increased at 6- and 16-months-of-age in both selected regions as compared to in cord blood values (p