An efficient method for recombineering GAL4 and QF drivers
Neural circuit mapping and manipulation are facilitated by independent control of gene expression in pre- and post-synaptic neurons. The GAL4/UAS and Q binary transcription systems have the potential to provide this capability. Of particular use in neural circuit mapping would be GAL4 and QF drivers...
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| Veröffentlicht in: | Fly (Austin, Tex.) Tex.), 2011-10, Vol.5 (4), p.371-378 |
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| creator | Stowers, R. Steven |
| description | Neural circuit mapping and manipulation are facilitated by independent control of gene expression in pre- and post-synaptic neurons. The GAL4/UAS and Q binary transcription systems have the potential to provide this capability. Of particular use in neural circuit mapping would be GAL4 and QF drivers specific for neurotransmitters and neurotransmitter receptors. Recently available Drosophila genomic BAC libraries make recombineering large genes including those specific for neurotransmitters and neurotransmitter receptors feasible. Here the functionality of cassettes that allow efficient recombineering of GAL4 and QF drivers based on kanamycin selection is demonstrated in Drosophila. The cassettes should, however, be generalizable for recombineering in other species. |
| doi_str_mv | 10.4161/fly.5.4.17560 |
| format | Article |
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| fulltext | fulltext |
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| ispartof | Fly (Austin, Tex.), 2011-10, Vol.5 (4), p.371-378 |
| issn | 1933-6934 1933-6942 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Binding Biology Bioscience Calcium Cancer Cell Chromosomes, Artificial, Bacterial - genetics Cycle Drosophila - genetics Drosophila Proteins - genetics Landes Methods & Technical Advances Mutagenesis, Insertional - methods Organogenesis Proteins Recombinant Fusion Proteins - genetics Recombination, Genetic Transcription Factors - genetics |
| title | An efficient method for recombineering GAL4 and QF drivers |
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