Cryopreservation of Goldfish Caudal Fin Explants using Glycerol as a Cryoprotecant
The objective of this study was to investigate the effect of glycerol on the cryopreservation fin explants of goldfish, Carassius auratus. Four different concentrations, 5, 10, 15, and 20% (v/v) of glycerol and a control were tested. These were prepared in Dulbecco's modified Eagle's mediu...
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| Veröffentlicht in: | Cryo-Letters 2011-01, Vol.32 (1), p.57-61 |
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| creator | Choresca, Casiano H. Gomez, Dennis K. Kim, Ji Hyung Han, Jee Eun Shin, Sang Phil Lee, Byeong Chun Park, Se Chang |
| description | The objective of this study was to investigate the effect of glycerol on the cryopreservation fin explants of goldfish, Carassius auratus. Four different concentrations, 5, 10, 15, and 20% (v/v) of glycerol and a control were tested. These were prepared in Dulbecco's modified
Eagle's medium with 20% (v/v) Fetal Bovine Serum. Attachment and outgrowing rates were monitored from day 3 to day 14. Results showed that fin explants cryopreserved in 20% concentration of glycerol was significantly higher (P |
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Eagle's medium with 20% (v/v) Fetal Bovine Serum. Attachment and outgrowing rates were monitored from day 3 to day 14. Results showed that fin explants cryopreserved in 20% concentration of glycerol was significantly higher (P<0.05) with a 100% attachment rate compared to 5, 10,
and 15% concentrations with 36.67, 84.19 and 86.51% attachment rate, respectively. Fin explants cryopreserved in 20% glycerol concentration also had significantly higher (P<0.05) outgrowth of cells (72.50%) than the other three concentrations on day 3. Moreover, a 100% outgrowth
of cells in all concentrations was achieved after 14 days of culture. No attachment and out growth of cells were observed in control group. Goldfish caudal fin explants cryopreserved in glycerol can produce live cells efficiently, regardless of concentration.</description><identifier>ISSN: 0143-2044</identifier><identifier>PMID: 21468454</identifier><language>eng</language><publisher>Lewes: Cryoletters</publisher><subject>Animal Fins - cytology ; Animal Fins - drug effects ; Animals ; Biological and medical sciences ; Caudal Fin ; Cell Adhesion ; Cell Count ; Cell Culture Techniques ; Cold Temperature ; Cryopreservation ; Cryoprotective Agents - pharmacology ; Diverse techniques ; Fish Explants ; Fundamental and applied biological sciences. Psychology ; Glycerol ; Glycerol - pharmacology ; Goldfish ; Molecular and cellular biology</subject><ispartof>Cryo-Letters, 2011-01, Vol.32 (1), p.57-61</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>289,314,780,784,4022</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23876462$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21468454$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choresca, Casiano H.</creatorcontrib><creatorcontrib>Gomez, Dennis K.</creatorcontrib><creatorcontrib>Kim, Ji Hyung</creatorcontrib><creatorcontrib>Han, Jee Eun</creatorcontrib><creatorcontrib>Shin, Sang Phil</creatorcontrib><creatorcontrib>Lee, Byeong Chun</creatorcontrib><creatorcontrib>Park, Se Chang</creatorcontrib><title>Cryopreservation of Goldfish Caudal Fin Explants using Glycerol as a Cryoprotecant</title><title>Cryo-Letters</title><addtitle>Cryo Letters</addtitle><description>The objective of this study was to investigate the effect of glycerol on the cryopreservation fin explants of goldfish, Carassius auratus. Four different concentrations, 5, 10, 15, and 20% (v/v) of glycerol and a control were tested. These were prepared in Dulbecco's modified
Eagle's medium with 20% (v/v) Fetal Bovine Serum. Attachment and outgrowing rates were monitored from day 3 to day 14. Results showed that fin explants cryopreserved in 20% concentration of glycerol was significantly higher (P<0.05) with a 100% attachment rate compared to 5, 10,
and 15% concentrations with 36.67, 84.19 and 86.51% attachment rate, respectively. Fin explants cryopreserved in 20% glycerol concentration also had significantly higher (P<0.05) outgrowth of cells (72.50%) than the other three concentrations on day 3. Moreover, a 100% outgrowth
of cells in all concentrations was achieved after 14 days of culture. No attachment and out growth of cells were observed in control group. Goldfish caudal fin explants cryopreserved in glycerol can produce live cells efficiently, regardless of concentration.</description><subject>Animal Fins - cytology</subject><subject>Animal Fins - drug effects</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Caudal Fin</subject><subject>Cell Adhesion</subject><subject>Cell Count</subject><subject>Cell Culture Techniques</subject><subject>Cold Temperature</subject><subject>Cryopreservation</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Diverse techniques</subject><subject>Fish Explants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycerol</subject><subject>Glycerol - pharmacology</subject><subject>Goldfish</subject><subject>Molecular and cellular biology</subject><issn>0143-2044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtqHDEQRXuREDt2fiFoE7Ia0Kv1WJrBngSMDcZeFzVqyZHRdE8ktcnk66N5GK-ihVSCw6mq-6E7p0yKBadSnnWfS3mhlGul7KfujDOpjOzlefewzLtpm33x-RVrnEYyBbKa0hBi-UWWOA-YyE0cyfWfbcKxFjKXOD6TVdo5n6dEsBAkR8lUvWvIZfcxYCr-y-m96J5urh-XPxa396ufy6vbRRTK1gXzolfeIldc2iGY4DlD69Zarbkxdk21dEjV0FM2MCFMUFpKLUL7Wsq8FBfd96O3df49-1JhE4vzqY3pp7mAUay3QvemkV9P5Lze-AG2OW4w7-AthgZ8OwFYHKaQcXSxvHPCaCUVb9zdkWsR-LEivExzHtuSEB24FgLsI98nDq-Cjww45YxaqoH1zMDgA86pQsUMz3-h6Ca8-o_wYDtcnDIG9HAEPxWUAea6L7T4ByuTk1M</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Choresca, Casiano H.</creator><creator>Gomez, Dennis K.</creator><creator>Kim, Ji Hyung</creator><creator>Han, Jee Eun</creator><creator>Shin, Sang Phil</creator><creator>Lee, Byeong Chun</creator><creator>Park, Se Chang</creator><general>Cryoletters</general><general>CryoLetters LLP</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20110101</creationdate><title>Cryopreservation of Goldfish Caudal Fin Explants using Glycerol as a Cryoprotecant</title><author>Choresca, Casiano H. ; Gomez, Dennis K. ; Kim, Ji Hyung ; Han, Jee Eun ; Shin, Sang Phil ; Lee, Byeong Chun ; Park, Se Chang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i369t-1e356e9a26249df8fe21a9cb76b2889b074ca06d501d1338f674473f01d901e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animal Fins - cytology</topic><topic>Animal Fins - drug effects</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Caudal Fin</topic><topic>Cell Adhesion</topic><topic>Cell Count</topic><topic>Cell Culture Techniques</topic><topic>Cold Temperature</topic><topic>Cryopreservation</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Diverse techniques</topic><topic>Fish Explants</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycerol</topic><topic>Glycerol - pharmacology</topic><topic>Goldfish</topic><topic>Molecular and cellular biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choresca, Casiano H.</creatorcontrib><creatorcontrib>Gomez, Dennis K.</creatorcontrib><creatorcontrib>Kim, Ji Hyung</creatorcontrib><creatorcontrib>Han, Jee Eun</creatorcontrib><creatorcontrib>Shin, Sang Phil</creatorcontrib><creatorcontrib>Lee, Byeong Chun</creatorcontrib><creatorcontrib>Park, Se Chang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cryo-Letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choresca, Casiano H.</au><au>Gomez, Dennis K.</au><au>Kim, Ji Hyung</au><au>Han, Jee Eun</au><au>Shin, Sang Phil</au><au>Lee, Byeong Chun</au><au>Park, Se Chang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryopreservation of Goldfish Caudal Fin Explants using Glycerol as a Cryoprotecant</atitle><jtitle>Cryo-Letters</jtitle><addtitle>Cryo Letters</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>32</volume><issue>1</issue><spage>57</spage><epage>61</epage><pages>57-61</pages><issn>0143-2044</issn><abstract>The objective of this study was to investigate the effect of glycerol on the cryopreservation fin explants of goldfish, Carassius auratus. Four different concentrations, 5, 10, 15, and 20% (v/v) of glycerol and a control were tested. These were prepared in Dulbecco's modified
Eagle's medium with 20% (v/v) Fetal Bovine Serum. Attachment and outgrowing rates were monitored from day 3 to day 14. Results showed that fin explants cryopreserved in 20% concentration of glycerol was significantly higher (P<0.05) with a 100% attachment rate compared to 5, 10,
and 15% concentrations with 36.67, 84.19 and 86.51% attachment rate, respectively. Fin explants cryopreserved in 20% glycerol concentration also had significantly higher (P<0.05) outgrowth of cells (72.50%) than the other three concentrations on day 3. Moreover, a 100% outgrowth
of cells in all concentrations was achieved after 14 days of culture. No attachment and out growth of cells were observed in control group. Goldfish caudal fin explants cryopreserved in glycerol can produce live cells efficiently, regardless of concentration.</abstract><cop>Lewes</cop><pub>Cryoletters</pub><pmid>21468454</pmid><tpages>5</tpages></addata></record> |
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| source | IngentaConnect; MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animal Fins - cytology Animal Fins - drug effects Animals Biological and medical sciences Caudal Fin Cell Adhesion Cell Count Cell Culture Techniques Cold Temperature Cryopreservation Cryoprotective Agents - pharmacology Diverse techniques Fish Explants Fundamental and applied biological sciences. Psychology Glycerol Glycerol - pharmacology Goldfish Molecular and cellular biology |
| title | Cryopreservation of Goldfish Caudal Fin Explants using Glycerol as a Cryoprotecant |
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