Cryopreservation of Goldfish Caudal Fin Explants using Glycerol as a Cryoprotecant

The objective of this study was to investigate the effect of glycerol on the cryopreservation fin explants of goldfish, Carassius auratus. Four different concentrations, 5, 10, 15, and 20% (v/v) of glycerol and a control were tested. These were prepared in Dulbecco's modified Eagle's mediu...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Cryo-Letters 2011-01, Vol.32 (1), p.57-61
Hauptverfasser:	Choresca, Casiano H., Gomez, Dennis K., Kim, Ji Hyung, Han, Jee Eun, Shin, Sang Phil, Lee, Byeong Chun, Park, Se Chang
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Fins - cytology Animal Fins - drug effects Animals Biological and medical sciences Caudal Fin Cell Adhesion Cell Count Cell Culture Techniques Cold Temperature Cryopreservation Cryoprotective Agents - pharmacology Diverse techniques Fish Explants Fundamental and applied biological sciences. Psychology Glycerol Glycerol - pharmacology Goldfish Molecular and cellular biology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The objective of this study was to investigate the effect of glycerol on the cryopreservation fin explants of goldfish, Carassius auratus. Four different concentrations, 5, 10, 15, and 20% (v/v) of glycerol and a control were tested. These were prepared in Dulbecco's modified Eagle's medium with 20% (v/v) Fetal Bovine Serum. Attachment and outgrowing rates were monitored from day 3 to day 14. Results showed that fin explants cryopreserved in 20% concentration of glycerol was significantly higher (P
ISSN:	0143-2044