Biosynthesis of Selenium-Modified tRNAs in Methanococcus vannielii

Selenium-containing nucleosides are natural components of several tRNA species in Methanococcus vannielii. In the present study, the incorporation of selenium from75SeO2- 3into these macromolecules was investigated in sonic extracts of M. vannielii. Nucleoside analysis of the75Se-labeled tRNAs from...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1990-08, Vol.87 (16), p.6345-6348
Hauptverfasser:	Politino, Michael, Tsai, Lin, Veres, Zsuzsa, Stadtman, Thressa C.
Format:	Artikel
Sprache:	eng
Schlagworte:	550501 > Metabolism-- Tracer Techniques Amino acids Amino Acids - metabolism Analytical, structural and metabolic biochemistry BACTERIA BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES Biochemistry Biological and medical sciences BIOSYNTHESIS Carbon Radioisotopes CHROMATOGRAPHY Cysteine - analogs & derivatives Cysteine - metabolism DAYS LIVING RADIOISOTOPES ELECTRON CAPTURE RADIOISOTOPES ELEMENTS Enzymes Euryarchaeota - metabolism EVEN-ODD NUCLEI Fundamental and applied biological sciences. Psychology INTERMEDIATE MASS NUCLEI ISOTOPE APPLICATIONS ISOTOPES Kinetics LIQUID COLUMN CHROMATOGRAPHY METABOLISM METALLOPROTEINS METHANOGENIC BACTERIA MICROORGANISMS NUCLEI NUCLEIC ACIDS Nucleosides ORGANIC COMPOUNDS Phenols PROTEINS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT Radioactive decay RADIOISOTOPES Reagents RNA Rna, ribonucleoproteins RNA, Transfer - isolation & purification RNA, Transfer - metabolism RNA, Transfer, Amino Acyl - biosynthesis SELENIUM Selenium - isolation & purification Selenium - metabolism SELENIUM 75 SELENIUM ISOTOPES Selenium Radioisotopes Selenocysteine Selenomethionine - metabolism SEMIMETALS SEPARATION PROCESSES SYNTHESIS 560300 > Chemicals Metabolism & Toxicology TRACER TECHNIQUES TRANSFER RNA
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Zusammenfassung:	Selenium-containing nucleosides are natural components of several tRNA species in Methanococcus vannielii. In the present study, the incorporation of selenium from75SeO2- 3into these macromolecules was investigated in sonic extracts of M. vannielii. Nucleoside analysis of the75Se-labeled tRNAs from these in vitro reaction mixtures demonstrated that the selenium was present in75Se-labeled nucleosides identical to the two naturally occurring 2-selenouridines produced in vivo. Incorporation of selenium into these nucleosides was ATP-dependent and was maximal after 20 min. Addition of O-acetylserine enhanced the activity 2- to 3-fold, implicating a role for selenocysteine in the reaction. Added L-selenocysteine could function as a selenium donor, but the D isomer and DL-selenomethionine were inactive. RPC-5 chromatography of bulk tRNA isolated from M. vannielii grown on75SeO2- 3separated five major species of seleno-tRNAs. The amino acid-accepting activity of these tRNAs was investigated.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.87.16.6345