Construction of a Dominant Negative Form of Human Hypoxia-Inducible Factor-2α
We constructed a dominant negative form of human hypoxia-inducible factor (HIF)-2α, HIF-2αDoN, which inhibited HIF transcriptional activity induced by hypoxia and by HIF-2α. HIF-2αDoN formed a complex with HIF-1β and interacted with DNA containing hypoxia response elements (HREs). Thus, the complex...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2010, Vol.74 (10), p.2100-2102 |
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| container_issue | 10 |
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| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 74 |
| creator | HIGASHIMURA, Yasuki YAMAJI, Ryoichi HARADA, Naoki NAKAO, Motoyuki NAKANO, Yoshihisa INUI, Hiroshi |
| description | We constructed a dominant negative form of human hypoxia-inducible factor (HIF)-2α, HIF-2αDoN, which inhibited HIF transcriptional activity induced by hypoxia and by HIF-2α. HIF-2αDoN formed a complex with HIF-1β and interacted with DNA containing hypoxia response elements (HREs). Thus, the complex appears to inhibit the binding of HIF-2 to HREs, and HIF-2αDoN might provide a useful therapeutic tool for HIF-2α-related diseases. |
| doi_str_mv | 10.1271/bbb.100208 |
| format | Article |
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Psychology</subject><subject>Genes, Dominant - genetics</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>hypoxia</subject><subject>hypoxia-inducible factor-1α</subject><subject>hypoxia-inducible factor-1β</subject><subject>hypoxia-inducible factor-2α</subject><subject>Protein Engineering - methods</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Isoforms - metabolism</subject><subject>Response Elements - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0M9KwzAcwPEgipvTiw8gvYggdCZp0iRHmX8myLzoufyaphJpk5m06h7LF_GZ7NjUi6dAfp_8CF-EjgmeEirIRVmWU4IxxXIHjUnGRJorJnbRGCuSp5JxMkIHMb5gPFxwso9GFCvGGFVjtJh5F7vQ6856l_g6geTKt9aB65KFeYbOvpnkxod2PZv3Lbhkvlr6Dwvpnat6bctmmIPufEjp1-ch2quhieZoe07Q083142ye3j_c3s0u71PNOO3SXGoiJRNMqZwbaYCqsqZS04wbDSB4KSSWtVBKcq1BU6ay3LAqqxgmRuJsgs42e5fBv_YmdkVrozZNA874PhYip0woSfggzzdSBx9jMHWxDLaFsCoILtb5iiFfsck34JPt2r5sTfVLf3oN4HQLIGpo6gBO2_jnMkEwY2RwfOOsq4d48O5DUxUdrBoffh5l_3zgG3ibiPM</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>HIGASHIMURA, Yasuki</creator><creator>YAMAJI, Ryoichi</creator><creator>HARADA, Naoki</creator><creator>NAKAO, Motoyuki</creator><creator>NAKANO, Yoshihisa</creator><creator>INUI, Hiroshi</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2010</creationdate><title>Construction of a Dominant Negative Form of Human Hypoxia-Inducible Factor-2α</title><author>HIGASHIMURA, Yasuki ; YAMAJI, Ryoichi ; HARADA, Naoki ; NAKAO, Motoyuki ; NAKANO, Yoshihisa ; INUI, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-68c1884749965e8ea29bf28c235ecaa75b7808f79985ccac24936e4d3d401e803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Basic Helix-Loop-Helix Transcription Factors - genetics</topic><topic>Basic Helix-Loop-Helix Transcription Factors - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>dominant negative form</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Dominant - genetics</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>hypoxia</topic><topic>hypoxia-inducible factor-1α</topic><topic>hypoxia-inducible factor-1β</topic><topic>hypoxia-inducible factor-2α</topic><topic>Protein Engineering - methods</topic><topic>Protein Isoforms - genetics</topic><topic>Protein Isoforms - metabolism</topic><topic>Response Elements - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HIGASHIMURA, Yasuki</creatorcontrib><creatorcontrib>YAMAJI, Ryoichi</creatorcontrib><creatorcontrib>HARADA, Naoki</creatorcontrib><creatorcontrib>NAKAO, Motoyuki</creatorcontrib><creatorcontrib>NAKANO, Yoshihisa</creatorcontrib><creatorcontrib>INUI, Hiroshi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HIGASHIMURA, Yasuki</au><au>YAMAJI, Ryoichi</au><au>HARADA, Naoki</au><au>NAKAO, Motoyuki</au><au>NAKANO, Yoshihisa</au><au>INUI, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of a Dominant Negative Form of Human Hypoxia-Inducible Factor-2α</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2010</date><risdate>2010</risdate><volume>74</volume><issue>10</issue><spage>2100</spage><epage>2102</epage><pages>2100-2102</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>We constructed a dominant negative form of human hypoxia-inducible factor (HIF)-2α, HIF-2αDoN, which inhibited HIF transcriptional activity induced by hypoxia and by HIF-2α. HIF-2αDoN formed a complex with HIF-1β and interacted with DNA containing hypoxia response elements (HREs). Thus, the complex appears to inhibit the binding of HIF-2 to HREs, and HIF-2αDoN might provide a useful therapeutic tool for HIF-2α-related diseases.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>20944429</pmid><doi>10.1271/bbb.100208</doi><tpages>3</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 2010, Vol.74 (10), p.2100-2102 |
| issn | 0916-8451 1347-6947 |
| language | eng |
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| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Freely Accessible Japanese Titles; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Basic Helix-Loop-Helix Transcription Factors - genetics Basic Helix-Loop-Helix Transcription Factors - metabolism Biological and medical sciences Cell Line, Tumor dominant negative form Fundamental and applied biological sciences. Psychology Genes, Dominant - genetics HEK293 Cells Humans hypoxia hypoxia-inducible factor-1α hypoxia-inducible factor-1β hypoxia-inducible factor-2α Protein Engineering - methods Protein Isoforms - genetics Protein Isoforms - metabolism Response Elements - genetics RNA, Messenger - genetics RNA, Messenger - metabolism |
| title | Construction of a Dominant Negative Form of Human Hypoxia-Inducible Factor-2α |
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