Construction of a Dominant Negative Form of Human Hypoxia-Inducible Factor-2α

We constructed a dominant negative form of human hypoxia-inducible factor (HIF)-2α, HIF-2αDoN, which inhibited HIF transcriptional activity induced by hypoxia and by HIF-2α. HIF-2αDoN formed a complex with HIF-1β and interacted with DNA containing hypoxia response elements (HREs). Thus, the complex...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2010, Vol.74 (10), p.2100-2102
Hauptverfasser:	HIGASHIMURA, Yasuki, YAMAJI, Ryoichi, HARADA, Naoki, NAKAO, Motoyuki, NAKANO, Yoshihisa, INUI, Hiroshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Basic Helix-Loop-Helix Transcription Factors - genetics Basic Helix-Loop-Helix Transcription Factors - metabolism Biological and medical sciences Cell Line, Tumor dominant negative form Fundamental and applied biological sciences. Psychology Genes, Dominant - genetics HEK293 Cells Humans hypoxia hypoxia-inducible factor-1α hypoxia-inducible factor-1β hypoxia-inducible factor-2α Protein Engineering - methods Protein Isoforms - genetics Protein Isoforms - metabolism Response Elements - genetics RNA, Messenger - genetics RNA, Messenger - metabolism
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Zusammenfassung:	We constructed a dominant negative form of human hypoxia-inducible factor (HIF)-2α, HIF-2αDoN, which inhibited HIF transcriptional activity induced by hypoxia and by HIF-2α. HIF-2αDoN formed a complex with HIF-1β and interacted with DNA containing hypoxia response elements (HREs). Thus, the complex appears to inhibit the binding of HIF-2 to HREs, and HIF-2αDoN might provide a useful therapeutic tool for HIF-2α-related diseases.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.100208