Magnetic moment degradation of nanowires in biological media: real-time monitoring with SQUID magnetometry

Magnetic nanoparticles are used throughout biology for applications from targeted drug and gene delivery to the labeling of cells. These nanoparticles typically react with the biological medium to which they are introduced, resulting in a diminished magnetic moment. The rate at which their magnetic moment is diminished limits their utility for targeting and can signal the unintended release of surface-functionalized biomolecules. A foreknowledge of the time-dependent degradation of the magnetic moment in a given medium can aid in the selection of the optimal buffering solution and in the prediction of a reasonable experimental time frame. With this goal in mind, we have developed a SQUID magnetometer based methodology for measuring the saturation magnetic moment of nanoparticles in real time while immersed in a biological medium. Measurements on Co and Ni nanowires in a variety of commonly used buffered salines demonstrated that the technique has the dynamic range and sensitivity to detect the rapid reduction in moment due to active corrosion as well as much more subtle changes from the formation of a passivating surface oxide layer. In order to correlate the magnetic moment reductions to these specific chemical processes, samples were additionally characterized using x-ray photoelectron spectroscopy, inductively coupled plasma spectroscopy and scanning electron microscopy. The most reactive buffers studied were found to be phosphate and carbonate based, which caused active corrosion of the Co nanowires but only a comparatively slow passivation of the Ni nanowires by oxidation.