Detection of 2,4,6-Trinitrotoluene-Utilizing Anaerobic Bacteria by ¹⁵N and ¹³C Incorporation
2,4,6-Trinitrotoluene (¹⁵N or ¹³C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [¹⁵N]TNT cultures (60, 163, and 168 bp). The 60-bp peak...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2010-03, Vol.76 (5), p.1695-1698 |
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| container_title | Applied and Environmental Microbiology |
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| creator | Gallagher, Erin M Young, Lily Y McGuinness, Lora M Kerkhof, Lee J |
| description | 2,4,6-Trinitrotoluene (¹⁵N or ¹³C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [¹⁵N]TNT cultures (60, 163, and 168 bp). The 60-bp peak was also present in the [¹³C]TNT cultures and was related to Lysobacter taiwanensis. |
| doi_str_mv | 10.1128/AEM.02274-09 |
| format | Article |
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Psychology</subject><subject>Geologic Sediments</subject><subject>Lysobacter - genetics</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nitrogen Isotopes - metabolism</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Trinitrotoluene - metabolism</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkD1vFDEQhi0EIkego4alQDTZMLZ3_dEgHUeASAEKcrU16_XeGe3ah3cPFDp-U5SCNj-FX4KjO76K0Yw0j555NYQ8pHBMKVPP5yfvjoExWZWgb5EZBa3KmnNxm8wAtC4Zq-CA3BvHTwBQgVB3yQEDUDTXjOArNzk7-RiK2BXsqDoS5XnywU8pTrHfuuDK5eR7_82HVTEP6FJsvC1eop1c8lg0F8X1j5_fr94XGNo8Xl8uitNgY9rEhDfe--ROh_3oHuz7IVm-PjlfvC3PPrw5XczPyo5qYGVTN1aprmPSddZqibKl1U3MpoVWMyuUkG3O3TIE5VorHWdW1YLaVnbKNvyQvNh5N9tmyIALU8LebJIfMF2YiN78vwl-bVbxi2GKM65YFjzbC1L8vHXjZAY_Wtf3GFzcjkZyXnNGRZXJR_-e-nPj91sz8HQP4Gix7xIG68e_HKuUFFpn7smOW_vV-qtPzuA4GHSDkcLUhgpdZ-bxjukwGlyl7Fl-ZEA5UAWqqjX_BcbonoM</recordid><startdate>201003</startdate><enddate>201003</enddate><creator>Gallagher, Erin M</creator><creator>Young, Lily Y</creator><creator>McGuinness, Lora M</creator><creator>Kerkhof, Lee J</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201003</creationdate><title>Detection of 2,4,6-Trinitrotoluene-Utilizing Anaerobic Bacteria by ¹⁵N and ¹³C Incorporation</title><author>Gallagher, Erin M ; Young, Lily Y ; McGuinness, Lora M ; Kerkhof, Lee J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f1902-b5bc88ff27efcc97a7d140081bd0d92c6867d200d2a08edc7e32c8561cd7f8cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Bacteria, Anaerobic - isolation & purification</topic><topic>Bacteria, Anaerobic - metabolism</topic><topic>Biological and medical sciences</topic><topic>Carbon Isotopes - metabolism</topic><topic>DNA Fingerprinting</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Geologic Sediments</topic><topic>Lysobacter - genetics</topic><topic>Methods</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nitrogen Isotopes - metabolism</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Trinitrotoluene - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gallagher, Erin M</creatorcontrib><creatorcontrib>Young, Lily Y</creatorcontrib><creatorcontrib>McGuinness, Lora M</creatorcontrib><creatorcontrib>Kerkhof, Lee J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gallagher, Erin M</au><au>Young, Lily Y</au><au>McGuinness, Lora M</au><au>Kerkhof, Lee J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of 2,4,6-Trinitrotoluene-Utilizing Anaerobic Bacteria by ¹⁵N and ¹³C Incorporation</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2010-03</date><risdate>2010</risdate><volume>76</volume><issue>5</issue><spage>1695</spage><epage>1698</epage><pages>1695-1698</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>2,4,6-Trinitrotoluene (¹⁵N or ¹³C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [¹⁵N]TNT cultures (60, 163, and 168 bp). The 60-bp peak was also present in the [¹³C]TNT cultures and was related to Lysobacter taiwanensis.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>20081008</pmid><doi>10.1128/AEM.02274-09</doi><tpages>4</tpages></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 2010-03, Vol.76 (5), p.1695-1698 |
| issn | 0099-2240 1098-5336 |
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| source | MEDLINE; NCBI_PubMed Central（免费）; ASM_美国微生物学会期刊; Alma/SFX Local Collection |
| subjects | Bacteria, Anaerobic - isolation & purification Bacteria, Anaerobic - metabolism Biological and medical sciences Carbon Isotopes - metabolism DNA Fingerprinting DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Geologic Sediments Lysobacter - genetics Methods Microbiology Molecular Sequence Data Nitrogen Isotopes - metabolism Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Trinitrotoluene - metabolism |
| title | Detection of 2,4,6-Trinitrotoluene-Utilizing Anaerobic Bacteria by ¹⁵N and ¹³C Incorporation |
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