Detection of 2,4,6-Trinitrotoluene-Utilizing Anaerobic Bacteria by ¹⁵N and ¹³C Incorporation

2,4,6-Trinitrotoluene (¹⁵N or ¹³C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [¹⁵N]TNT cultures (60, 163, and 168 bp). The 60-bp peak...

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Veröffentlicht in:	Applied and Environmental Microbiology 2010-03, Vol.76 (5), p.1695-1698
Hauptverfasser:	Gallagher, Erin M, Young, Lily Y, McGuinness, Lora M, Kerkhof, Lee J
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria, Anaerobic - isolation & purification Bacteria, Anaerobic - metabolism Biological and medical sciences Carbon Isotopes - metabolism DNA Fingerprinting DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Geologic Sediments Lysobacter - genetics Methods Microbiology Molecular Sequence Data Nitrogen Isotopes - metabolism Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Trinitrotoluene - metabolism
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Beschreibung
Zusammenfassung:	2,4,6-Trinitrotoluene (¹⁵N or ¹³C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [¹⁵N]TNT cultures (60, 163, and 168 bp). The 60-bp peak was also present in the [¹³C]TNT cultures and was related to Lysobacter taiwanensis.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.02274-09