BchY-Based Degenerate Primers Target All Types of Anoxygenic Photosynthetic Bacteria in a Single PCR

To detect anoxygenic bacteria containing either type 1 or type 2 photosynthetic reaction centers in a single PCR, we designed a degenerate primer set based on the bchY gene. The new primers were validated in silico using the GenBank nucleotide database as well as by PCR on pure strains and environme...

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Veröffentlicht in:	Applied and Environmental Microbiology 2009-12, Vol.75 (23), p.7556-7559
Hauptverfasser:	Yutin, Natalya, Suzuki, Marcelino T, Rosenberg, Mira, Rotem, Denisse, Madigan, Michael T, Süling, Jörg, Imhoff, Johannes F, Béjà, Oded
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Anaerobiosis Bacteria Bacteria - genetics Bacteria - isolation & purification Bacteria - metabolism Bacterial Proteins - genetics Biological and medical sciences Deoxyribonucleic acid DNA DNA Primers - genetics Fundamental and applied biological sciences. Psychology Methods Microbiology Molecular Sequence Data Photosynthesis Phylogeny Polymerase Chain Reaction - methods Sequence Alignment Validity
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Beschreibung
Zusammenfassung:	To detect anoxygenic bacteria containing either type 1 or type 2 photosynthetic reaction centers in a single PCR, we designed a degenerate primer set based on the bchY gene. The new primers were validated in silico using the GenBank nucleotide database as well as by PCR on pure strains and environmental DNA.
ISSN:	0099-2240 1098-5336 1098-6596
DOI:	10.1128/AEM.01014-09