Transformation of Arabidopsis with Plant-Derived DNA Sequences Necessary for Selecting Transformants and Driving an Objective Gene

Most genetically modified plants are made with antibiotic-resistant genes and promoters derived from plant pathogens. To promote public acceptance, we have adapted a selectable marker and promoters all of which were derived from Arabidopsis thaliana to transformation. We have employed the gene for a...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-04, Vol.73 (4), p.936-938
Hauptverfasser: AHMAD, Aftab, KAJI, Izumi, MURAKAMI, Yukiko, FUNATO, Nana, OGAWA, Takeshi, SHIMIZU, Masanori, NIWA, Yasuo, KOBAYASHI, Hirokazu
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Sprache:eng
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Zusammenfassung:Most genetically modified plants are made with antibiotic-resistant genes and promoters derived from plant pathogens. To promote public acceptance, we have adapted a selectable marker and promoters all of which were derived from Arabidopsis thaliana to transformation. We have employed the gene for acetolactate synthase with its own promoter as a selectable marker, and the actin 2 gene promoter to strongly express an objective gene.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.80725