Development of Multiplex PCR Assays for Detection of Enterotoxigenic Escherichia coli Colonization Factors and Toxins

Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnost...

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Veröffentlicht in:	Journal of Clinical Microbiology 2009-04, Vol.47 (4), p.1218-1220
Hauptverfasser:	Rodas, Claudia, Iniguez, Volga, Qadri, Firdausi, Wiklund, Gudrun, Svennerholm, Ann-Mari, Sjöling, Åsa
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Toxins Bacterial Toxins - genetics Bacteriology Bangladesh Biological and medical sciences Bolivia Enterotoxigenic Escherichia coli Enterotoxigenic Escherichia coli - genetics Enterotoxigenic Escherichia coli - pathogenicity Escherichia coli Escherichia coli Proteins Escherichia coli Proteins - genetics Fundamental and applied biological sciences. Psychology genetics Humans methods Microbiology Microbiology in the medical area Mikrobiologi inom det medicinska området Miscellaneous pathogenicity Polymerase Chain Reaction Polymerase Chain Reaction - methods Virulence Factors Virulence Factors - genetics
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Zusammenfassung:	Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnostic laboratories that have access to PCR.
ISSN:	0095-1137 1098-660X 1098-660X
DOI:	10.1128/JCM.00316-09