Inhibition of Restenosis Formation Without Compromising Reendothelialization as a Potential Solution to Thrombosis Following Angioplasty?
Stent thrombosis remains an important problem after the implantation of different stent types. A potential solution to this problem may be vasoactive agents with dual effects on different cell types like C-type natriuretic peptide (CNP). Therefore, in vitro and in vivo effects of CNP were investigat...
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| Veröffentlicht in: | Endothelium (New York, N.Y.) N.Y.), 2008, Vol.15 (1-2), p.85-92 |
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| creator | Fuchs, Alexandra Theresia Kuehnl, Andreas Pelisek, Jaroslav Rolland, Pierre Henri Mekkaoui, Choukri Netz, Heinrich Nikol, Sigrid |
| description | Stent thrombosis remains an important problem after the implantation of different stent types. A potential solution to this problem may be vasoactive agents with dual effects on different cell types like C-type natriuretic peptide (CNP). Therefore, in vitro and in vivo effects of CNP were investigated in a porcine restenotic model. Gene transfer of CNP in cultures of porcine vascular cells revealed up to 30% reduction of growth of smooth muscle cells (p |
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A potential solution to this problem may be vasoactive agents with dual effects on different cell types like C-type natriuretic peptide (CNP). Therefore, in vitro and in vivo effects of CNP were investigated in a porcine restenotic model. Gene transfer of CNP in cultures of porcine vascular cells revealed up to 30% reduction of growth of smooth muscle cells (p<.05), but no suppression of endothelial growth using CNP. Applied in vivo, angiography revealed a trend of reduced restenosis formation in balloon-injured porcine arteries treated with CNP gene or β-galactosidase (β-Gal) control gene after three months (2.59 ± 2.04-fold reduction, p = n.s.). Histologically, morphometry revealed significantly reduced neointima formation after treatment with CNP plasmid (7.26 ± 1.44-fold reduction, p < .05). Evans blue staining demonstrated complete endothelial repair already 3 weeks after intervention using CNP. Transfer of CNP gene resulted in a significant inhibition of neointima formation without compromising endothelial repair. Therefore, use of the CNP gene may offer a solution to suppress restenosis formation while preventing subacute or late thrombosis.</description><identifier>ISSN: 1062-3329</identifier><identifier>EISSN: 1029-2373</identifier><identifier>DOI: 10.1080/10623320802092484</identifier><identifier>PMID: 18568948</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Angiography ; Angioplasty, Balloon - adverse effects ; Animals ; Arteries - cytology ; C-Type Natriuretic Peptide ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Coloring Agents - metabolism ; Constriction, Pathologic ; Disease Models, Animal ; DNA - genetics ; Endothelial Cells - cytology ; Endothelial Cells - drug effects ; Endothelium, Vascular - physiopathology ; Evans Blue - metabolism ; Gene Expression ; Gene Transfer Techniques ; Immunohistochemistry ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - drug effects ; Natriuretic Peptide, C-Type - administration & dosage ; Natriuretic Peptide, C-Type - genetics ; Neointima Formation ; Nonviral Gene Transfer ; Reendothelialization ; Subacute Thrombosis ; Swine ; Thrombosis - prevention & control ; Time Factors ; Transfection ; Tunica Intima - drug effects</subject><ispartof>Endothelium (New York, N.Y.), 2008, Vol.15 (1-2), p.85-92</ispartof><rights>2008 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-e4c7e00f79b2eb3f957dc9b0cdf230b2ae28f151c2a70e26c093f13a4c303a9c3</citedby><cites>FETCH-LOGICAL-c404t-e4c7e00f79b2eb3f957dc9b0cdf230b2ae28f151c2a70e26c093f13a4c303a9c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/10623320802092484$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/10623320802092484$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,4010,27900,27901,27902,59620,60409,61194,61375</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18568948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fuchs, Alexandra Theresia</creatorcontrib><creatorcontrib>Kuehnl, Andreas</creatorcontrib><creatorcontrib>Pelisek, Jaroslav</creatorcontrib><creatorcontrib>Rolland, Pierre Henri</creatorcontrib><creatorcontrib>Mekkaoui, Choukri</creatorcontrib><creatorcontrib>Netz, Heinrich</creatorcontrib><creatorcontrib>Nikol, Sigrid</creatorcontrib><title>Inhibition of Restenosis Formation Without Compromising Reendothelialization as a Potential Solution to Thrombosis Following Angioplasty?</title><title>Endothelium (New York, N.Y.)</title><addtitle>Endothelium</addtitle><description>Stent thrombosis remains an important problem after the implantation of different stent types. A potential solution to this problem may be vasoactive agents with dual effects on different cell types like C-type natriuretic peptide (CNP). Therefore, in vitro and in vivo effects of CNP were investigated in a porcine restenotic model. Gene transfer of CNP in cultures of porcine vascular cells revealed up to 30% reduction of growth of smooth muscle cells (p<.05), but no suppression of endothelial growth using CNP. Applied in vivo, angiography revealed a trend of reduced restenosis formation in balloon-injured porcine arteries treated with CNP gene or β-galactosidase (β-Gal) control gene after three months (2.59 ± 2.04-fold reduction, p = n.s.). Histologically, morphometry revealed significantly reduced neointima formation after treatment with CNP plasmid (7.26 ± 1.44-fold reduction, p < .05). Evans blue staining demonstrated complete endothelial repair already 3 weeks after intervention using CNP. Transfer of CNP gene resulted in a significant inhibition of neointima formation without compromising endothelial repair. Therefore, use of the CNP gene may offer a solution to suppress restenosis formation while preventing subacute or late thrombosis.</description><subject>Angiography</subject><subject>Angioplasty, Balloon - adverse effects</subject><subject>Animals</subject><subject>Arteries - cytology</subject><subject>C-Type Natriuretic Peptide</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Coloring Agents - metabolism</subject><subject>Constriction, Pathologic</subject><subject>Disease Models, Animal</subject><subject>DNA - genetics</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - drug effects</subject><subject>Endothelium, Vascular - physiopathology</subject><subject>Evans Blue - metabolism</subject><subject>Gene Expression</subject><subject>Gene Transfer Techniques</subject><subject>Immunohistochemistry</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Natriuretic Peptide, C-Type - administration & dosage</subject><subject>Natriuretic Peptide, C-Type - genetics</subject><subject>Neointima Formation</subject><subject>Nonviral Gene Transfer</subject><subject>Reendothelialization</subject><subject>Subacute Thrombosis</subject><subject>Swine</subject><subject>Thrombosis - prevention & control</subject><subject>Time Factors</subject><subject>Transfection</subject><subject>Tunica Intima - drug effects</subject><issn>1062-3329</issn><issn>1029-2373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF9rFDEUxYNYbF39AL7IPPk2epPMv6AgZWm1UKhoxcchk0k6KZncNclQ1m_gt262uyBF6FMuh9855BxC3lB4T6GDDxQaxjnLJwPBqq56Rk4oMFEy3vLnu7thZQbEMXkZ4y0A1JzxF-SYdnXTiao7IX8v_GQHmyz6Ak3xXcekPUYbi3MMs3zQf9k04ZKKNc6bgLON1t9kUvsR06Sdlc7-2ZMyFrL4hjkiZbX4gW550BMW11O2Dodk5_BuF3LqbyxunIxp-_kVOTLSRf368K7Iz_Oz6_XX8vLqy8X69LJUFVSp1JVqNYBpxcD0wI2o21GJAdRoGIeBSc06Q2uqmGxBs0aB4IZyWSkOXArFV-TdPjd3-b3kvn1upLRz0mtcYt8Ixmuad10RugdVwBiDNv0m2FmGbU-h3-3f_7d_9rw9hC_DrMd_jsPgGfi0B6w3u4HvMLixT3LrMJggvbKx50_lf3xkn7R0aVIy6P4Wl-DzcE_87h5JEalu</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Fuchs, Alexandra Theresia</creator><creator>Kuehnl, Andreas</creator><creator>Pelisek, Jaroslav</creator><creator>Rolland, Pierre Henri</creator><creator>Mekkaoui, Choukri</creator><creator>Netz, Heinrich</creator><creator>Nikol, Sigrid</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2008</creationdate><title>Inhibition of Restenosis Formation Without Compromising Reendothelialization as a Potential Solution to Thrombosis Following Angioplasty?</title><author>Fuchs, Alexandra Theresia ; Kuehnl, Andreas ; Pelisek, Jaroslav ; Rolland, Pierre Henri ; Mekkaoui, Choukri ; Netz, Heinrich ; Nikol, Sigrid</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-e4c7e00f79b2eb3f957dc9b0cdf230b2ae28f151c2a70e26c093f13a4c303a9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Angiography</topic><topic>Angioplasty, Balloon - adverse effects</topic><topic>Animals</topic><topic>Arteries - cytology</topic><topic>C-Type Natriuretic Peptide</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Coloring Agents - metabolism</topic><topic>Constriction, Pathologic</topic><topic>Disease Models, Animal</topic><topic>DNA - genetics</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - drug effects</topic><topic>Endothelium, Vascular - physiopathology</topic><topic>Evans Blue - metabolism</topic><topic>Gene Expression</topic><topic>Gene Transfer Techniques</topic><topic>Immunohistochemistry</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Natriuretic Peptide, C-Type - administration & dosage</topic><topic>Natriuretic Peptide, C-Type - genetics</topic><topic>Neointima Formation</topic><topic>Nonviral Gene Transfer</topic><topic>Reendothelialization</topic><topic>Subacute Thrombosis</topic><topic>Swine</topic><topic>Thrombosis - prevention & control</topic><topic>Time Factors</topic><topic>Transfection</topic><topic>Tunica Intima - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fuchs, Alexandra Theresia</creatorcontrib><creatorcontrib>Kuehnl, Andreas</creatorcontrib><creatorcontrib>Pelisek, Jaroslav</creatorcontrib><creatorcontrib>Rolland, Pierre Henri</creatorcontrib><creatorcontrib>Mekkaoui, Choukri</creatorcontrib><creatorcontrib>Netz, Heinrich</creatorcontrib><creatorcontrib>Nikol, Sigrid</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Endothelium (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fuchs, Alexandra Theresia</au><au>Kuehnl, Andreas</au><au>Pelisek, Jaroslav</au><au>Rolland, Pierre Henri</au><au>Mekkaoui, Choukri</au><au>Netz, Heinrich</au><au>Nikol, Sigrid</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of Restenosis Formation Without Compromising Reendothelialization as a Potential Solution to Thrombosis Following Angioplasty?</atitle><jtitle>Endothelium (New York, N.Y.)</jtitle><addtitle>Endothelium</addtitle><date>2008</date><risdate>2008</risdate><volume>15</volume><issue>1-2</issue><spage>85</spage><epage>92</epage><pages>85-92</pages><issn>1062-3329</issn><eissn>1029-2373</eissn><abstract>Stent thrombosis remains an important problem after the implantation of different stent types. A potential solution to this problem may be vasoactive agents with dual effects on different cell types like C-type natriuretic peptide (CNP). Therefore, in vitro and in vivo effects of CNP were investigated in a porcine restenotic model. Gene transfer of CNP in cultures of porcine vascular cells revealed up to 30% reduction of growth of smooth muscle cells (p<.05), but no suppression of endothelial growth using CNP. Applied in vivo, angiography revealed a trend of reduced restenosis formation in balloon-injured porcine arteries treated with CNP gene or β-galactosidase (β-Gal) control gene after three months (2.59 ± 2.04-fold reduction, p = n.s.). Histologically, morphometry revealed significantly reduced neointima formation after treatment with CNP plasmid (7.26 ± 1.44-fold reduction, p < .05). Evans blue staining demonstrated complete endothelial repair already 3 weeks after intervention using CNP. Transfer of CNP gene resulted in a significant inhibition of neointima formation without compromising endothelial repair. Therefore, use of the CNP gene may offer a solution to suppress restenosis formation while preventing subacute or late thrombosis.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>18568948</pmid><doi>10.1080/10623320802092484</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Taylor & Francis Journals Complete |
| subjects | Angiography Angioplasty, Balloon - adverse effects Animals Arteries - cytology C-Type Natriuretic Peptide Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured Coloring Agents - metabolism Constriction, Pathologic Disease Models, Animal DNA - genetics Endothelial Cells - cytology Endothelial Cells - drug effects Endothelium, Vascular - physiopathology Evans Blue - metabolism Gene Expression Gene Transfer Techniques Immunohistochemistry Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Natriuretic Peptide, C-Type - administration & dosage Natriuretic Peptide, C-Type - genetics Neointima Formation Nonviral Gene Transfer Reendothelialization Subacute Thrombosis Swine Thrombosis - prevention & control Time Factors Transfection Tunica Intima - drug effects |
| title | Inhibition of Restenosis Formation Without Compromising Reendothelialization as a Potential Solution to Thrombosis Following Angioplasty? |
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