Novel promoter sequence required for inductive expression of the Aspergillus nidulans endoglucanase gene eglA

Novel promoter sequence required for inductive expression of the Aspergillus nidulans endoglucanase gene eglA

Expression of the eglA gene, encoding for a major endoglucanse EG A in Aspergillus nidulans, is induced by cellulose and cellobiose, but not by xylose. This suggests that induction is independent of XlnR, a transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus. Mutational an...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2008-02, Vol.72 (2), p.312-320
Hauptverfasser: Endo, Y.(Nagoya Univ. (Japan)), Yokoyama, M, Morimoto, M, Shirai, K, Chikamatsu, G, Kato, N, Tsukagoshi, N, Kato, M, Kobayashi, T
Format: Artikel
Sprache:eng
Schlagworte:
ASPERGILLUS NIDULANS
Aspergillus nidulans - enzymology
Base Sequence
Biological and medical sciences
BIOMASA
BIOMASS
BIOMASSE
Cellulase - biosynthesis
Cellulase - genetics
CELLULOSE
Cellulose - metabolism
CELULOSA
DNA Primers
DNA, Fungal
eglA
Enzyme Induction
Fundamental and applied biological sciences. Psychology
GENE
GENES
induction
Molecular Sequence Data
MUTACION
Mutagenesis, Site-Directed
MUTATION
NUCLEOTIDE SEQUENCE
Promoter Regions, Genetic
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
TRANSCRIPCION
TRANSCRIPTION
upstream activating sequence
Xylans - metabolism
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Zusammenfassung:Expression of the eglA gene, encoding for a major endoglucanse EG A in Aspergillus nidulans, is induced by cellulose and cellobiose, but not by xylose. This suggests that induction is independent of XlnR, a transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus. Mutational analysis of the eglA promoter was performed to identify the novel cis-element responsible for XlnR-independent induction. The region spanning - 153 to -138 (CCGTACCTTTTTAGGA), designated CeRE(Cellulose Responsive Element), was found to be the upstream activating element essential to inductive expression of the eglA gene.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.70278