Development of Immunochromatography Strip-Test Using Nanocolloidal Gold-Antibody Probe for the Rapid Detection of Aflatoxin B1 in Grain and Feed Samples
An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed o...
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| Veröffentlicht in: | Journal of microbiology and biotechnology 2007-10, Vol.17 (10), p.1629-1637 |
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| creator | Shim, W.B. (Gyeongsang National University, Jinju, Republic of Korea) Yang, Z.Y. (Gyeongsang National University, Jinju, Republic of Korea) Kim, J.S. (Gyeongsang National University, Jinju, Republic of Korea) Kim, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) Kang, S.J. (Gyeongsang National University, Jinju, Republic of Korea) Woo, G.J. (Korea Food and Drug Administration, Seoul, Republic of Korea) Chung, Y.C. (Jinju International University, Jinju, Republic of Korea) Eremin, Sergei A. (Moscow State University, Moscow, Russia) Chung, D.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: dhchung@gnu.ac.kr |
| description | An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. |
| format | Article |
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(Gyeongsang National University, Jinju, Republic of Korea) ; Yang, Z.Y. (Gyeongsang National University, Jinju, Republic of Korea) ; Kim, J.S. (Gyeongsang National University, Jinju, Republic of Korea) ; Kim, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) ; Kang, S.J. (Gyeongsang National University, Jinju, Republic of Korea) ; Woo, G.J. (Korea Food and Drug Administration, Seoul, Republic of Korea) ; Chung, Y.C. (Jinju International University, Jinju, Republic of Korea) ; Eremin, Sergei A. (Moscow State University, Moscow, Russia) ; Chung, D.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: dhchung@gnu.ac.kr</creator><creatorcontrib>Shim, W.B. (Gyeongsang National University, Jinju, Republic of Korea) ; Yang, Z.Y. (Gyeongsang National University, Jinju, Republic of Korea) ; Kim, J.S. (Gyeongsang National University, Jinju, Republic of Korea) ; Kim, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) ; Kang, S.J. (Gyeongsang National University, Jinju, Republic of Korea) ; Woo, G.J. (Korea Food and Drug Administration, Seoul, Republic of Korea) ; Chung, Y.C. (Jinju International University, Jinju, Republic of Korea) ; Eremin, Sergei A. (Moscow State University, Moscow, Russia) ; Chung, D.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: dhchung@gnu.ac.kr</creatorcontrib><description>An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC.</description><identifier>ISSN: 1017-7825</identifier><identifier>PMID: 18156778</identifier><language>eng</language><publisher>Seoul: Korean Society for Applied Microbiology</publisher><subject>aflatoxin B1 ; Aflatoxin B1 - analysis ; Animal Feed - analysis ; Animals ; Antibodies, Bacterial ; Antibodies, Monoclonal - immunology ; Antigens, Fungal ; Chromatography - economics ; Chromatography - methods ; Chromatography, High Pressure Liquid - methods ; Edible Grain - chemistry ; Gold Colloid - immunology ; Immunoassay - economics ; Immunoassay - methods ; immunochromatography strip test ; Metal Nanoparticles ; Mice ; Mice, Inbred BALB C ; MICOTOXINAS ; monoclonal antibody ; MYCOTOXINE ; MYCOTOXINS ; nanocolloidal gold ; Sensitivity and Specificity</subject><ispartof>Journal of microbiology and biotechnology, 2007-10, Vol.17 (10), p.1629-1637</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19198396$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18156778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shim, W.B. (Gyeongsang National University, Jinju, Republic of Korea)</creatorcontrib><creatorcontrib>Yang, Z.Y. (Gyeongsang National University, Jinju, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, J.S. (Gyeongsang National University, Jinju, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, J.Y. (Gyeongsang National University, Jinju, Republic of Korea)</creatorcontrib><creatorcontrib>Kang, S.J. (Gyeongsang National University, Jinju, Republic of Korea)</creatorcontrib><creatorcontrib>Woo, G.J. (Korea Food and Drug Administration, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Chung, Y.C. (Jinju International University, Jinju, Republic of Korea)</creatorcontrib><creatorcontrib>Eremin, Sergei A. (Moscow State University, Moscow, Russia)</creatorcontrib><creatorcontrib>Chung, D.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: dhchung@gnu.ac.kr</creatorcontrib><title>Development of Immunochromatography Strip-Test Using Nanocolloidal Gold-Antibody Probe for the Rapid Detection of Aflatoxin B1 in Grain and Feed Samples</title><title>Journal of microbiology and biotechnology</title><addtitle>J Microbiol Biotechnol</addtitle><description>An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC.</description><subject>aflatoxin B1</subject><subject>Aflatoxin B1 - analysis</subject><subject>Animal Feed - analysis</subject><subject>Animals</subject><subject>Antibodies, Bacterial</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Fungal</subject><subject>Chromatography - economics</subject><subject>Chromatography - methods</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Edible Grain - chemistry</subject><subject>Gold Colloid - immunology</subject><subject>Immunoassay - economics</subject><subject>Immunoassay - methods</subject><subject>immunochromatography strip test</subject><subject>Metal Nanoparticles</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>MICOTOXINAS</subject><subject>monoclonal antibody</subject><subject>MYCOTOXINE</subject><subject>MYCOTOXINS</subject><subject>nanocolloidal gold</subject><subject>Sensitivity and Specificity</subject><issn>1017-7825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0E1OwzAQBeAsQLQUjgDyhmUkO2nsZFlaWioqQP1ZVxN73Bo5ceS4iN6E4xLUIjZvNp_ek-Yi6jPKRCzyJOtF1237QSlnSc6voh7LWcaFyPvR9wQ_0bqmwjoQp8m8qg61k3vvKghu56HZH8kqeNPEa2wD2bSm3pFX6Iyz1hkFlsycVfGoDqZ06kjevSuRaOdJ2CNZQmMUmWBAGYyrfydG2nbVX6Ymj4x0OfPQJdSKTBEVWUHVWGxvoksNtsXb8x1Em-nTevwcL95m8_FoEeuEsxDLAkGkkgqalKioHHJRaCYTLjgTZaESrrUQDJUCmomkEBmVmGMpOU07NUwH0f2ptzmUFapt400F_rj9e1EHHs4AWglWe6ilaf9dwYo8LXjn7k5Og9vCznfmZZlQmlNKhzlLfwAnOHlN</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Shim, W.B. (Gyeongsang National University, Jinju, Republic of Korea)</creator><creator>Yang, Z.Y. (Gyeongsang National University, Jinju, Republic of Korea)</creator><creator>Kim, J.S. (Gyeongsang National University, Jinju, Republic of Korea)</creator><creator>Kim, J.Y. (Gyeongsang National University, Jinju, Republic of Korea)</creator><creator>Kang, S.J. (Gyeongsang National University, Jinju, Republic of Korea)</creator><creator>Woo, G.J. (Korea Food and Drug Administration, Seoul, Republic of Korea)</creator><creator>Chung, Y.C. (Jinju International University, Jinju, Republic of Korea)</creator><creator>Eremin, Sergei A. (Moscow State University, Moscow, Russia)</creator><creator>Chung, D.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: dhchung@gnu.ac.kr</creator><general>Korean Society for Applied Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20071001</creationdate><title>Development of Immunochromatography Strip-Test Using Nanocolloidal Gold-Antibody Probe for the Rapid Detection of Aflatoxin B1 in Grain and Feed Samples</title><author>Shim, W.B. (Gyeongsang National University, Jinju, Republic of Korea) ; Yang, Z.Y. (Gyeongsang National University, Jinju, Republic of Korea) ; Kim, J.S. (Gyeongsang National University, Jinju, Republic of Korea) ; Kim, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) ; Kang, S.J. (Gyeongsang National University, Jinju, Republic of Korea) ; Woo, G.J. (Korea Food and Drug Administration, Seoul, Republic of Korea) ; Chung, Y.C. (Jinju International University, Jinju, Republic of Korea) ; Eremin, Sergei A. (Moscow State University, Moscow, Russia) ; Chung, D.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: dhchung@gnu.ac.kr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f261t-c9ea73c0702bed0c4679f1c267617b9d26ff771edda05729750ce8ebc603c2643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>aflatoxin B1</topic><topic>Aflatoxin B1 - analysis</topic><topic>Animal Feed - analysis</topic><topic>Animals</topic><topic>Antibodies, Bacterial</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, Fungal</topic><topic>Chromatography - economics</topic><topic>Chromatography - methods</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Edible Grain - chemistry</topic><topic>Gold Colloid - immunology</topic><topic>Immunoassay - economics</topic><topic>Immunoassay - methods</topic><topic>immunochromatography strip test</topic><topic>Metal Nanoparticles</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>MICOTOXINAS</topic><topic>monoclonal antibody</topic><topic>MYCOTOXINE</topic><topic>MYCOTOXINS</topic><topic>nanocolloidal gold</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shim, W.B. (Gyeongsang National University, Jinju, Republic of Korea)</creatorcontrib><creatorcontrib>Yang, Z.Y. (Gyeongsang National University, Jinju, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, J.S. (Gyeongsang National University, Jinju, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, J.Y. (Gyeongsang National University, Jinju, Republic of Korea)</creatorcontrib><creatorcontrib>Kang, S.J. (Gyeongsang National University, Jinju, Republic of Korea)</creatorcontrib><creatorcontrib>Woo, G.J. (Korea Food and Drug Administration, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Chung, Y.C. (Jinju International University, Jinju, Republic of Korea)</creatorcontrib><creatorcontrib>Eremin, Sergei A. (Moscow State University, Moscow, Russia)</creatorcontrib><creatorcontrib>Chung, D.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: dhchung@gnu.ac.kr</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Journal of microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shim, W.B. (Gyeongsang National University, Jinju, Republic of Korea)</au><au>Yang, Z.Y. (Gyeongsang National University, Jinju, Republic of Korea)</au><au>Kim, J.S. (Gyeongsang National University, Jinju, Republic of Korea)</au><au>Kim, J.Y. (Gyeongsang National University, Jinju, Republic of Korea)</au><au>Kang, S.J. (Gyeongsang National University, Jinju, Republic of Korea)</au><au>Woo, G.J. (Korea Food and Drug Administration, Seoul, Republic of Korea)</au><au>Chung, Y.C. (Jinju International University, Jinju, Republic of Korea)</au><au>Eremin, Sergei A. (Moscow State University, Moscow, Russia)</au><au>Chung, D.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: dhchung@gnu.ac.kr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of Immunochromatography Strip-Test Using Nanocolloidal Gold-Antibody Probe for the Rapid Detection of Aflatoxin B1 in Grain and Feed Samples</atitle><jtitle>Journal of microbiology and biotechnology</jtitle><addtitle>J Microbiol Biotechnol</addtitle><date>2007-10-01</date><risdate>2007</risdate><volume>17</volume><issue>10</issue><spage>1629</spage><epage>1637</epage><pages>1629-1637</pages><issn>1017-7825</issn><abstract>An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC.</abstract><cop>Seoul</cop><pub>Korean Society for Applied Microbiology</pub><pmid>18156778</pmid><tpages>9</tpages></addata></record> |
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| ispartof | Journal of microbiology and biotechnology, 2007-10, Vol.17 (10), p.1629-1637 |
| issn | 1017-7825 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | aflatoxin B1 Aflatoxin B1 - analysis Animal Feed - analysis Animals Antibodies, Bacterial Antibodies, Monoclonal - immunology Antigens, Fungal Chromatography - economics Chromatography - methods Chromatography, High Pressure Liquid - methods Edible Grain - chemistry Gold Colloid - immunology Immunoassay - economics Immunoassay - methods immunochromatography strip test Metal Nanoparticles Mice Mice, Inbred BALB C MICOTOXINAS monoclonal antibody MYCOTOXINE MYCOTOXINS nanocolloidal gold Sensitivity and Specificity |
| title | Development of Immunochromatography Strip-Test Using Nanocolloidal Gold-Antibody Probe for the Rapid Detection of Aflatoxin B1 in Grain and Feed Samples |
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