Development of Immunochromatography Strip-Test Using Nanocolloidal Gold-Antibody Probe for the Rapid Detection of Aflatoxin B1 in Grain and Feed Samples

An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed o...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2007-10, Vol.17 (10), p.1629-1637
Hauptverfasser:	Shim, W.B. (Gyeongsang National University, Jinju, Republic of Korea), Yang, Z.Y. (Gyeongsang National University, Jinju, Republic of Korea), Kim, J.S. (Gyeongsang National University, Jinju, Republic of Korea), Kim, J.Y. (Gyeongsang National University, Jinju, Republic of Korea), Kang, S.J. (Gyeongsang National University, Jinju, Republic of Korea), Woo, G.J. (Korea Food and Drug Administration, Seoul, Republic of Korea), Chung, Y.C. (Jinju International University, Jinju, Republic of Korea), Eremin, Sergei A. (Moscow State University, Moscow, Russia), Chung, D.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: dhchung@gnu.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	aflatoxin B1 Aflatoxin B1 - analysis Animal Feed - analysis Animals Antibodies, Bacterial Antibodies, Monoclonal - immunology Antigens, Fungal Chromatography - economics Chromatography - methods Chromatography, High Pressure Liquid - methods Edible Grain - chemistry Gold Colloid - immunology Immunoassay - economics Immunoassay - methods immunochromatography strip test Metal Nanoparticles Mice Mice, Inbred BALB C MICOTOXINAS monoclonal antibody MYCOTOXINE MYCOTOXINS nanocolloidal gold Sensitivity and Specificity
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Zusammenfassung:	An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC.
ISSN:	1017-7825