Characterization of Shiga Toxin-Producing Escherichia coli Isolates Associated with Two Multistate Food-Borne Outbreaks That Occurred in 2006

Shiga toxin-producing Escherichia coli isolates from two 2006 outbreaks were compared to other O157:H7 isolates for virulence genotype, biofilm formation, and stress responses. Spinach- and lettuce-related-outbreak strains had similar pulsed-field gel electrophoresis patterns, and all carried both s...

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Veröffentlicht in:	Applied and Environmental Microbiology 2008-02, Vol.74 (4), p.1268-1272
Hauptverfasser:	Uhlich, G.A, Sinclair, J.R, Warren, N.G, Chmielecki, W.A, Fratamico, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence biofilm Biofilms Biofilms - growth & development Biological and medical sciences Colitis - epidemiology Colitis - microbiology Disease Outbreaks DNA Primers - genetics E coli Electrophoresis, Gel, Pulsed-Field Epidemics Escherichia coli Escherichia coli O157 Escherichia coli O157:H7 food contamination Food Microbiology foodborne illness Fundamental and applied biological sciences. Psychology gel electrophoresis Genes Genotype Humans Lactuca - microbiology Lettuce microbial contamination microbial genetics Microbiology Molecular Sequence Data Pennsylvania - epidemiology Sequence Analysis, DNA Sequence Homology Serotyping Shiga toxin Shiga Toxin - genetics Shiga Toxin - metabolism Shiga-Toxigenic Escherichia coli - genetics Shiga-Toxigenic Escherichia coli - metabolism Shiga-Toxigenic Escherichia coli - pathogenicity spinach Spinacia oleracea - microbiology strains Vegetables Virulence
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Zusammenfassung:	Shiga toxin-producing Escherichia coli isolates from two 2006 outbreaks were compared to other O157:H7 isolates for virulence genotype, biofilm formation, and stress responses. Spinach- and lettuce-related-outbreak strains had similar pulsed-field gel electrophoresis patterns, and all carried both stx₂ and stx₂c variant genes. Cooperative biofilm formation involving an E. coli O157:H7 strain and a non-O157:H7 strain was also demonstrated.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.01618-07