Quantitative PCR for Detection and Enumeration of Genetic Markers of Bovine Fecal Pollution

Accurate assessment of health risks associated with bovine (cattle) fecal pollution requires a reliable host-specific genetic marker and a rapid quantification method. We report the development of quantitative PCR assays for the detection of two recently described bovine feces-specific genetic marke...

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Veröffentlicht in:	Applied and Environmental Microbiology 2008-02, Vol.74 (3), p.745-752
Hauptverfasser:	Shanks, Orin C, Atikovic, Emina, Blackwood, A. Denene, Lu, Jingrang, Noble, Rachel T, Domingo, Jorge Santo, Seifring, Shawn, Sivaganesan, Mano, Haugland, Richard A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteria Bacteroides thetaiotaomicron Biological and medical sciences Cattle DNA Primers DNA, Bacterial - analysis DNA, Bacterial - isolation & purification DNA, Ribosomal - analysis Feces Feces - microbiology Fundamental and applied biological sciences. Psychology Genes, rRNA Genetic Markers - genetics Genetics Markov Chains Methods Microbiology Monte Carlo Method Monte Carlo simulation Plasmids - genetics Pollution Polymerase Chain Reaction - methods Species Specificity Water Pollution - analysis
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Zusammenfassung:	Accurate assessment of health risks associated with bovine (cattle) fecal pollution requires a reliable host-specific genetic marker and a rapid quantification method. We report the development of quantitative PCR assays for the detection of two recently described bovine feces-specific genetic markers and a method for the enumeration of these markers using a Markov chain Monte Carlo approach. Both assays exhibited a range of quantification from 25 to 2 x 10⁶ copies of target DNA, with a coefficient of variation of
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.01843-07