Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay

A competitive inhibition enzyme-linked immunosorbent assay (CI ELISA) was developed to detect antibody to Babesia equi. One hundred fifty-four equine serum samples from 19 countries were tested for antibody to B. equi by the complement fixation test and by CI ELISA. The CI ELISA and complement fixat...

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Veröffentlicht in:	Journal of Clinical Microbiology 1991-09, Vol.29 (9), p.2056-2058
Hauptverfasser:	Knowles, D.P. Jr. (Animal Disease Research Unit, USDA-ARS, Pullman, WA), Perryman, L.E, Kappmeyer, L.S, Hennager, S.G
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal Antibodies, Protozoan - analysis ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES Babesia - immunology BABESIA EQUI Babesiosis - diagnosis Babesiosis - immunology Binding, Competitive Biological and medical sciences CABALLOS CHEVAL Complement Fixation Tests DISTRIBUCION NATURAL DISTRIBUTION NATURELLE ELISA Enzyme-Linked Immunosorbent Assay - methods Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Fundamental immunology Horse Diseases - diagnosis Horse Diseases - immunology Horses LIQUIDE BIOLOGIQUE LIQUIDOS CORPORALES Molecular immunology PROTEINAS PROTEINE Protozoan Proteins - immunology PRUEBAS DE FIJACION DEL COMPLEMENTO REACTION DE DEVIATION DU COMPLEMENT Techniques TEST ELISA
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Zusammenfassung:	A competitive inhibition enzyme-linked immunosorbent assay (CI ELISA) was developed to detect antibody to Babesia equi. One hundred fifty-four equine serum samples from 19 countries were tested for antibody to B. equi by the complement fixation test and by CI ELISA. The CI ELISA and complement fixation test results agreed in 94% (144) of the serum samples tested. The 10 discrepant serum samples were retested and analyzed for ability to immunoprecipitate in vitro translation products from B. equi merozoite mRNA. Five discrepant results were clearly resolved in favor of the CI ELISA, and the remaining five discrepancies were not definitively resolved
ISSN:	0095-1137 1098-660X
DOI:	10.1128/jcm.29.9.2056-2058.1991