Identification of the circadian transcriptome in adult mouse skeletal muscle

1 Department of Physiology, University of Kentucky, Lexington, Kentucky 2 School of Kinesiology, University of Illinois Chicago, Chicago 3 Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois 4 Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 5 The Genomics Institute of the Novartis Research Foundation, San Diego, California Circadian rhythms are approximate 24-h behavioral and physiological cycles that function to prepare an organism for daily environmental changes. The basic clock mechanism is a network of transcriptional-translational feedback loops that drive rhythmic expression of genes over a 24-h period. The objectives of this study were to identify transcripts with a circadian pattern of expression in adult skeletal muscle and to determine the effect of the Clock mutation on gene expression. Expression profiling on muscle samples collected every 4 h for 48 h was performed. Using COSOPT, we identified a total of 215 transcripts as having a circadian pattern of expression. Real-time PCR results verified the circadian expression of the core clock genes, Bmal1 , Per2 , and Cry2 . Annotation revealed cycling genes were involved in a range of biological processes including transcription, lipid metabolism, protein degradation, ion transport, and vesicular trafficking. The tissue specificity of the skeletal muscle circadian transcriptome was highlighted by the presence of known muscle-specific genes such as Myod1 , Ucp3 , Atrogin1 ( Fbxo32 ), and Myh1 (myosin heavy chain IIX). Expression profiling was also performed on muscle from the Clock mutant mouse and sarcomeric genes such as actin and titin, and many mitochondrial genes were significantly downregulated in the muscle of Clock mutant mice. Defining the circadian transcriptome in adult skeletal muscle and identifying the significant alterations in gene expression that occur in muscle of the Clock mutant mouse provide the basis for understanding the role of circadian rhythms in the daily maintenance of skeletal muscle. circadian rhythms; gene expression; MyoD; Clock mutant