Effects of enamel matrix derivative and transforming growth factor-beta1 on human periodontal ligament fibroblasts

The objective of this study was to evaluate the effects of enamel matrix derivative (EMD), transforming growth factor-beta1 (TGF-beta1), and a combination of both factors (EMD+TGF-beta1) on periodontal ligament (PDL) fibroblasts. Human PDL fibroblasts were obtained from three adult patients with a c...

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Veröffentlicht in:	Journal of clinical periodontology 2007-06, Vol.34 (6), p.514
Hauptverfasser:	Rodrigues, Thaisângela L S, Marchesan, Julie T, Coletta, Ricardo D, Novaes, Jr, Arthur B, Grisi, Márcio F de M, Souza, Sérgio L S, Taba, Jr, Mário, Palioto, Daniela B
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Alkaline Phosphatase - drug effects Antimetabolites Biocompatible Materials - administration & dosage Biocompatible Materials - pharmacology Bromodeoxyuridine Calcification, Physiologic - drug effects Cell Adhesion - drug effects Cell Proliferation - drug effects Cells, Cultured Dental Enamel Proteins - administration & dosage Dental Enamel Proteins - pharmacology Drug Combinations Fibroblasts - cytology Fibroblasts - drug effects Humans Periodontal Ligament - cytology Periodontal Ligament - drug effects Proteins - drug effects Time Factors Transforming Growth Factor beta1 - administration & dosage Transforming Growth Factor beta1 - pharmacology Up-Regulation - drug effects Wound Healing - drug effects
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Zusammenfassung:	The objective of this study was to evaluate the effects of enamel matrix derivative (EMD), transforming growth factor-beta1 (TGF-beta1), and a combination of both factors (EMD+TGF-beta1) on periodontal ligament (PDL) fibroblasts. Human PDL fibroblasts were obtained from three adult patients with a clinically healthy periodontium, using the explant technique. The effects of EMD, TGF-beta1, or a combination of both were analysed on PDL cell proliferation, adhesion, wound healing, and total protein synthesis, and on alkaline phosphatase (ALP) activity and bone-like nodule formation. Treatment with EMD for 4, 7, and 10 days increased cell proliferation significantly compared with the negative control (p
ISSN:	0303-6979