Comparison of Tests for Detection of β-Lactamase-Producing Staphylococci

Comparison of Tests for Detection of β-Lactamase-Producing Staphylococci

Penicillin resistance identification tests are important in veterinary medicine. Six enzyme assays and a PCR test were compared for the detection of β-lactamase production or the β-lactamase gene in 175 staphylococcal isolates. We conclude that the PCR test and two nitrocefin-based assays can be rec...

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Veröffentlicht in:Journal of Clinical Microbiology 2007-06, Vol.45 (6), p.2031-2033
Hauptverfasser: Pitkälä, A, Salmikivi, L, Bredbacka, P, Myllyniemi, A.-L, Koskinen, M.T
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bacteriology
beta-Lactamases - biosynthesis
beta-Lactamases - genetics
Biological and medical sciences
Cattle
Cattle Diseases - microbiology
Cephalosporins - pharmacology
Clinical Veterinary Microbiology
Coagulase - metabolism
Colorimetry - methods
Fundamental and applied biological sciences. Psychology
Indicators and Reagents - pharmacology
Infectious diseases
Mastitis, Bovine - microbiology
Medical sciences
Microbial Sensitivity Tests
Microbiology
Miscellaneous
Polymerase Chain Reaction - methods
Sensitivity and Specificity
Staphylococcal Infections - microbiology
Staphylococcal Infections - veterinary
Staphylococcus - drug effects
Staphylococcus - enzymology
Staphylococcus - genetics
Staphylococcus - isolation & purification
Staphylococcus aureus - classification
Staphylococcus aureus - enzymology
Staphylococcus aureus - genetics
Staphylococcus aureus - isolation & purification
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Zusammenfassung:Penicillin resistance identification tests are important in veterinary medicine. Six enzyme assays and a PCR test were compared for the detection of β-lactamase production or the β-lactamase gene in 175 staphylococcal isolates. We conclude that the PCR test and two nitrocefin-based assays can be recommended for routine clinical use.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00621-07