Identification and Quantification of Methanogenic Archaea in Adult Chicken Ceca

By using molecular methods for the identification and quantification of methanogenic archaea in adult chicken ceca, 16S rRNA genes of 11 different phylotypes, 10 of which were 99% similar to Methanobrevibacter woesei, were found. Methanogen populations, as assessed by cultivation, and the 16S rRNA c...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Applied and Environmental Microbiology 2007-01, Vol.73 (1), p.353-356
Hauptverfasser:	Saengkerdsub, Suwat, Anderson, Robin C, Wilkinson, Heather H, Kim, Woo-Kyun, Nisbet, David J, Ricke, Steven C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Archaea Archaea - classification Archaea - genetics Archaea - isolation & purification Archaea - metabolism Biological and medical sciences Cecum - microbiology Chickens - microbiology DNA, Archaeal - analysis Female Fundamental and applied biological sciences. Psychology Methane - metabolism Methanobrevibacter Methanobrevibacter - classification Methanobrevibacter - genetics Methanobrevibacter - isolation & purification Methanobrevibacter - metabolism Microbial Ecology Microbiology Molecular Sequence Data Molecular structure Poultry Ribonucleic acid RNA RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Taxonomy
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	By using molecular methods for the identification and quantification of methanogenic archaea in adult chicken ceca, 16S rRNA genes of 11 different phylotypes, 10 of which were 99% similar to Methanobrevibacter woesei, were found. Methanogen populations, as assessed by cultivation, and the 16S rRNA copy number were between 6.38 and 8.23 cells/g (wet weight) and 5.50 and 7.19 log₁₀/g (wet weight), respectively.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.01931-06