Effects of Ca2+ channel antagonists on nerve stimulation-induced and ischemia-induced myocardial interstitial acetylcholine release in cats

1 Department of Cardiovascular Dynamics, Advanced Medical Engineering Center, National Cardiovascular Center Research Institute and 2 Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Osaka, Japan Submitted 17 February 2006 ; accepted in final form 7 June 2006 Although an axoplasmic Ca 2+ increase is associated with an exocytotic acetylcholine (ACh) release from the parasympathetic postganglionic nerve endings, the role of voltage-dependent Ca 2+ channels in ACh release in the mammalian cardiac parasympathetic nerve is not clearly understood. Using a cardiac microdialysis technique, we examined the effects of Ca 2+ channel antagonists on vagal nerve stimulation- and ischemia-induced myocardial interstitial ACh releases in anesthetized cats. The vagal stimulation-induced ACh release [22.4 nM (SD 10.6), n = 7] was significantly attenuated by local administration of an N-type Ca 2+ channel antagonist -conotoxin GVIA [11.7 nM (SD 5.8), n = 7, P = 0.0054], or a P/Q-type Ca 2+ channel antagonist -conotoxin MVIIC [3.8 nM (SD 2.3), n = 6, P = 0.0002] but not by local administration of an L-type Ca 2+ channel antagonist verapamil [23.5 nM (SD 6.0), n = 5, P = 0.758]. The ischemia-induced myocardial interstitial ACh release [15.0 nM (SD 8.3), n = 8] was not attenuated by local administration of the L-, N-, or P/Q-type Ca 2+ channel antagonists, by inhibition of Na + /Ca 2+ exchange, or by blockade of inositol 1,4,5-trisphosphate [Ins(1,4,5)P 3 ] receptor but was significantly suppressed by local administration of gadolinium [2.8 nM (SD 2.6), n = 6, P = 0.0283]. In conclusion, stimulation-induced ACh release from the cardiac postganglionic nerves depends on the N- and P/Q-type Ca 2+ channels (with a dominance of P/Q-type) but probably not on the L-type Ca 2+ channels in cats. In contrast, ischemia-induced ACh release depends on nonselective cation channels or cation-selective stretch activated channels but not on L-, N-, or P/Q type Ca 2+ channels, Na + /Ca 2+ exchange, or Ins(1,4,5)P 3 receptor-mediated pathway. cardiac microdialysis; -conotoxin GVIA; -conotoxin MVIIC; KB-R7943; verapamil; vagal stimulation Address for reprint requests and other correspondence: T. Kawada, Dept. of Cardiovascular Dynamics, Advanced Medical Engineering Center, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan (e-mail: torukawa{at}res.ncvc.go.jp )