Presence of a Novel DNA Methylation Enzyme in Methicillin-Resistant Staphylococcus aureus Isolates Associated with Pig Farming Leads to Uninterpretable Results in Standard Pulsed-Field Gel Electrophoresis Analysis

Genomic DNA from methicillin-resistant Staphylococcus aureus isolates recovered from pigs and their caretakers proved resistant to SmaI digestion, leading to uninterpretable results in standard pulsed-field gel electrophoresis. This is the result of a yet unknown restriction/methylation system in th...

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Veröffentlicht in:	Journal of Clinical Microbiology 2006-05, Vol.44 (5), p.1875-1876
Hauptverfasser:	Bens, Corina C.P.M, Voss, Andreas, Klaassen, Corné H.W
Format:	Artikel
Sprache:	eng
Schlagworte:	5-Methylcytosine - metabolism Animal Husbandry Animals Bacteriology Base Sequence Biological and medical sciences Deoxyribonucleases, Type II Site-Specific DNA Methylation DNA, Bacterial - genetics DNA, Bacterial - isolation & purification DNA, Bacterial - metabolism Electrophoresis, Gel, Pulsed-Field Fundamental and applied biological sciences. Psychology Humans Infectious diseases Medical sciences Methicillin Resistance Microbiology Miscellaneous Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Staphylococcus aureus - metabolism Sus scrofa - microbiology
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Beschreibung
Zusammenfassung:	Genomic DNA from methicillin-resistant Staphylococcus aureus isolates recovered from pigs and their caretakers proved resistant to SmaI digestion, leading to uninterpretable results in standard pulsed-field gel electrophoresis. This is the result of a yet unknown restriction/methylation system in the genus Staphylococcus with the recognition sequence CCNGG.
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.44.5.1875-1876.2006