Diagnostic Oligonucleotide Microarray Fingerprinting of Bacillus Isolates

A genome-independent microarray and new statistical techniques were used to genotype Bacillus strains and quantitatively compare DNA fingerprints with the known taxonomy of the genus. A synthetic DNA standard was used to understand process level variability and lead to recommended standard operating...

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Veröffentlicht in:	Journal of Clinical Microbiology 2006, Vol.44 (1), p.244-250
Hauptverfasser:	Chandler, Darrell P, Alferov, Oleg, Chernov, Boris, Daly, Don S, Golova, Julia, Perov, Alexander, Protic, Miroslava, Robison, Richard, Schipma, Matthew, White, Amanda, Willse, Alan
Format:	Artikel
Sprache:	eng
Schlagworte:	BACILLUS Bacillus - classification Bacillus - genetics Bacillus - isolation & purification BACILLUS CEREUS Bacterial Typing Techniques Bacteriology BASIC BIOLOGICAL SCIENCES Biological and medical sciences DNA DNA Fingerprinting - methods fingerprinting Fundamental and applied biological sciences. Psychology Genome, Bacterial Infectious diseases Medical sciences microarrays microbes Microbiology Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis - methods OLIGONUCLEOTIDES Phylogeny RECOMMENDATIONS STRAINS
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Beschreibung
Zusammenfassung:	A genome-independent microarray and new statistical techniques were used to genotype Bacillus strains and quantitatively compare DNA fingerprints with the known taxonomy of the genus. A synthetic DNA standard was used to understand process level variability and lead to recommended standard operating procedures for microbial forensics and clinical diagnostics.
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.44.1.244-250.2006