Fluorescence resonance energy transfer between a quantum dot donor and a dye acceptor attached to DNA

We show that direct coupling of a dye-labelled DNA (acceptor) to a quantum dot (QD) donor significantly reduces the donor-acceptor distance and improves the FRET efficiency: a highly efficient FRET (approximately 88%) at a low acceptor-to-donor ratio of 2 has been achieved at the single-molecule lev...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2005-10 (38), p.4807
Hauptverfasser:	Zhou, Dejian, Piper, Joe D, Abell, Chris, Klenerman, David, Kang, Dae-Joon, Ying, Liming
Format:	Artikel
Sprache:	eng
Schlagworte:	Cadmium Compounds - chemistry Coloring Agents - chemistry DNA - chemistry Fluorescence Resonance Energy Transfer - methods Quantum Dots Selenium Compounds - chemistry Sensitivity and Specificity Structure-Activity Relationship Sulfhydryl Compounds - chemistry Sulfides - chemistry Time Factors Zinc Compounds - chemistry
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Beschreibung
Zusammenfassung:	We show that direct coupling of a dye-labelled DNA (acceptor) to a quantum dot (QD) donor significantly reduces the donor-acceptor distance and improves the FRET efficiency: a highly efficient FRET (approximately 88%) at a low acceptor-to-donor ratio of 2 has been achieved at the single-molecule level.
ISSN:	1359-7345
DOI:	10.1039/b508911e