6-Azauracil inhibition of GTP biosynthesis in Saccharomyces cerevisiae

The addition of 6-azauracil to the growth medium causes a strong reduction of the GTP level in the nucleotide pool of Saccharomyces cerevisiae. In-vitro experiments show a strong inhibition of IMP dehydrogenase activity by 6-azaUMP explaining the preceeding effect. PPR2 mutants, previously character...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Current genetics 1992-07, Vol.22 (1), p.9-11
Hauptverfasser:	Exinger, F. (Centre National da la Recherche Scientifique, Strasbourg (France). IBMC), Lacroute, F
Format:	Artikel
Sprache:	eng
Schlagworte:	6-azauracil Antimetabolites - pharmacology Biological and medical sciences BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS Classical genetics, quantitative genetics, hybrids Fundamental and applied biological sciences. Psychology Genetics & Heredity Genetics of eukaryotes. Biological and molecular evolution GTP level GUANIDINA GUANIDINE GUANIDINES Guanosine Triphosphate - antagonists & inhibitors Guanosine Triphosphate - biosynthesis IMP dehydrogenase Life Sciences & Biomedicine OXIDOREDUCTASES OXIDORREDUCTASAS OXYDOREDUCTASE SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - metabolism Science & Technology Thallophyta, bryophyta Uracil - analogs & derivatives Uracil - pharmacology Vegetals
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The addition of 6-azauracil to the growth medium causes a strong reduction of the GTP level in the nucleotide pool of Saccharomyces cerevisiae. In-vitro experiments show a strong inhibition of IMP dehydrogenase activity by 6-azaUMP explaining the preceeding effect. PPR2 mutants, previously characterized by an increased sensitivity to 6-azauracil compared to the wild-type, are specifically susceptible to the lowering of the GTP pool, and are able to grow in presence of 6-azauracil when guanine is added to the medium.
ISSN:	0172-8083 1432-0983
DOI:	10.1007/bf00351735