Synthesis of hydroquinone-α-glucoside by α-glucosidase from baker's yeast

Synthesis of hydroquinone-α-glucoside by α-glucosidase from baker's yeast

Hydroquinone-alpha-glucoside was synthesised from hydroquinone and maltose as glucosyl donor by transglucosylation in a water system with alpha-glucosidase from baker's yeast. Only one phenolic -OH group was alpha-anomer-selectively glucosylated. The optimum conditions for transglucosylation re...

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Veröffentlicht in:Biotechnology letters 2005-04, Vol.27 (8), p.551-554
Hauptverfasser: PRODANOVIC, Radivoje M, MILOSAVIE, Nenad B, SLADIC, Dusan, TANJA CIRKOVIC VELICKOVIC, VUJCIC, Zoran
Format: Artikel
Sprache:eng
Schlagworte:
alpha-Glucosidases - isolation & purification
alpha-Glucosidases - metabolism
Biological and medical sciences
Biotechnology
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Fundamental and applied biological sciences. Psychology
Glucosides - analysis
Glucosides - biosynthesis
Glycosylation
Hydrogen-Ion Concentration
Hydroquinones - metabolism
Magnetic Resonance Spectroscopy - methods
Maltose - metabolism
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae Proteins - isolation & purification
Saccharomyces cerevisiae Proteins - metabolism
Temperature
Time Factors
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Zusammenfassung:Hydroquinone-alpha-glucoside was synthesised from hydroquinone and maltose as glucosyl donor by transglucosylation in a water system with alpha-glucosidase from baker's yeast. Only one phenolic -OH group was alpha-anomer-selectively glucosylated. The optimum conditions for transglucosylation reaction were at 30 degrees C for 20 h with 50 mM: hydroquinone and 1.5 M: maltose in 100 mM: sodium citrate/phosphate buffer at pH 5.5. The glucoside was obtained at 0.6 mg/ml with a 4.6% molar yield with respect to hydroquinone.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-005-2880-9