Identification of Two-Component Regulatory Genes Involved in o-Xylene Degradation by Rhodococcus sp. Strain DK17

Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions,...

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Veröffentlicht in:	The journal of microbiology 2005-02, Vol.43 (1), p.49
Hauptverfasser:	Kim, D.K. (Yonsei University, Seoul, Republic of Korea), Chae, J.C. (Rutgers University, New Brunswick, New Jersey, USA), Zylstra, Gerben J. (Rutgers University, New Brunswick, New Jersey, USA), Sohn, H.Y. (Andong National University, Andong, Republic of Korea), Kwon, G.S. (Andong National University, Andong, Republic of Korea), Kim, E.B. (Yonsei University, Seoul, Republic of Korea), E-mail: eungbin@yonsei.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	alkylbenzenes Amino Acid Motifs Amino Acid Sequence Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Binding Sites - genetics Biodegradation, Environmental Conserved Sequence DNA, Bacterial - genetics Genes, Bacterial Genes, Regulator Molecular Sequence Data RHODOCOCCUS (BACTERIA) Rhodococcus - genetics Rhodococcus - metabolism Sequence Homology, Amino Acid Signal Transduction - genetics two-component signal transduction proteins Xylenes - metabolism
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Zusammenfassung:	Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.
ISSN:	1225-8873 1976-3794