Structural and functional analysis of pTB6 from Bifidobacterium longum

The complete nucleolide sequence for pTB6 (3,624 bp) from Bifidobacterium longum was determined. This plasmid is 95% homologous in nucleotide (nuc) sequence, and also 92% in RepB as sequence, to rolling circle replication (RCR) plasmids pKJ36 and pB44. suggesting that pTB6 replicates by the rolling...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005-02, Vol.69 (2), p.422-425
Hauptverfasser:	Tanaka, K. (Kyoto Pharmaceutical Univ. (Japan)), Samura, K, Kano, Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence BIFIDOBACTERIUM Bifidobacterium - genetics Bifidobacterium longum Biological and medical sciences Chromosome Mapping Chromosomes, Bacterial DNA Fundamental and applied biological sciences. Psychology GENES Genes, Bacterial GENETIC TRANSFORMATION GENETIC VECTORS high-efficient transformation immobile plasmid IMMOBILIZATION Molecular Sequence Data NUCLEOTIDE SEQUENCE nucleotide sequence of pTB6 PLASMIDS Plasmids - genetics RepB and oriV REPLICATION Sequence Homology, Nucleic Acid
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Zusammenfassung:	The complete nucleolide sequence for pTB6 (3,624 bp) from Bifidobacterium longum was determined. This plasmid is 95% homologous in nucleotide (nuc) sequence, and also 92% in RepB as sequence, to rolling circle replication (RCR) plasmids pKJ36 and pB44. suggesting that pTB6 replicates by the rolling circle mechanism. The putative MembB, MobA, and protein encoding from orf (Orf) I detected were nonessential for plasmid replication. We constructed an immobile shuttle vector from pTB6 and pUC18, which transformed B. longum with a high efficiency of 2.5 ×10E+6 trans-formants/Microg DNA.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.69.422