A short form of the tick-borne encephalitis virus NS3 protein

Using monoclonal antibodies to the tick-borne encephalitis virus (TBE) nonstructural protein NS3 two forms of this protein were revealed in TBE-infected mammalian cells: a full-length form (69 kDa) and a short form (49 kDa) which has not been observed before and was called NS3′. Recombinant plasmids...

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Veröffentlicht in:	FEBS letters 1992-02, Vol.297 (1), p.67-69
Hauptverfasser:	Pugachev, K.V., Nomokonova, N.Yu, Morozova, O.V., Pletnev, A.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biochemistry & Molecular Biology Biological and medical sciences Biophysics Blotting, Western Cell Biology Cells, Cultured Cleavage DNA, Viral - genetics Electrophoresis, Polyacrylamide Gel Encephalitis Viruses, Tick-Borne - metabolism Flavivirus form Fundamental and applied biological sciences. Psychology Humans Life Sciences & Biomedicine Microbiology Molecular Sequence Data monoclonal antibodies Nonstructural protein NS3 protein Plasmids Protein Biosynthesis Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA Helicases RNA, Messenger - genetics Science & Technology Serine Endopeptidases Swine tick-borne encephalitis virus Transcription, Genetic Viral Nonstructural Proteins Viral Proteins - genetics Virology
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Beschreibung
Zusammenfassung:	Using monoclonal antibodies to the tick-borne encephalitis virus (TBE) nonstructural protein NS3 two forms of this protein were revealed in TBE-infected mammalian cells: a full-length form (69 kDa) and a short form (49 kDa) which has not been observed before and was called NS3′. Recombinant plasmids were constructed and various fragments of the TBE NS3 gene were expressed in rabbit reticulocyte lysate. By analyzing immune precipitates of 35S-labeled translation products, we could monitor and localize internal cleavage of NS3, due to which the NS3′ protein was generated.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(92)80329-F