HPLC-based activity profiling of Salvia miltiorrhiza for MAO A and iNOS inhibitory activities

Abstract In the course of a screening of plant extracts for potential CNS and anti-inflammatory activities, a dichloromethane extract of SALVIA MILTIORRHIZA showed a pronounced inhibitory effect on recombinant monoamine oxidase A (MAO A) and on inducible NO synthase (iNOS) induction in Raw 267.4 cel...

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Veröffentlicht in:	Planta medica 2004-10, Vol.70 (10), p.909-913
Hauptverfasser:	Dittmann, K, Gerhauser, C, Klimo, K, Hamburger, M
Format:	Artikel
Sprache:	eng
Schlagworte:	15,16-dihydrotanshinone amine oxidase (flavin-containing) Animals Anti-Inflammatory Agents, Non-Steroidal - administration & dosage Anti-Inflammatory Agents, Non-Steroidal - pharmacology Anti-Inflammatory Agents, Non-Steroidal - therapeutic use Biological and medical sciences cell lines chemical structure diterpenoids enzyme inhibitors Enzyme Inhibitors - administration & dosage Enzyme Inhibitors - pharmacology Enzyme Inhibitors - therapeutic use General pharmacology high performance liquid chromatography human health Humans Inhibitory Concentration 50 Lamiaceae Macrophages - drug effects Macrophages - enzymology Medical sciences Mice Monoamine Oxidase - drug effects nitric oxide synthase Nitric Oxide Synthase - antagonists & inhibitors Nitric Oxide Synthase - drug effects Nitric Oxide Synthase Type II Original Paper Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments phytochemicals Phytotherapy plant extracts Plant Extracts - administration & dosage Plant Extracts - pharmacology Plant Extracts - therapeutic use Plant Roots Salvia miltiorrhiza tanshinones
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Zusammenfassung:	Abstract In the course of a screening of plant extracts for potential CNS and anti-inflammatory activities, a dichloromethane extract of SALVIA MILTIORRHIZA showed a pronounced inhibitory effect on recombinant monoamine oxidase A (MAO A) and on inducible NO synthase (iNOS) induction in Raw 267.4 cells. With the aid of HPLC-based profiling techniques, activities could be linked, to a significant extent, to peaks of tanshinone-type diterpenoids, four of which were subsequently isolated. The IC 50 of the most active compound, 15,16-dihydrotanshinone I, on human recombinant MAO A was at 23 μM, and 2.4 μM on lipopolysaccharide-mediated iNOS induction in Raw 267.4 cells.
ISSN:	0032-0943 1439-0221
DOI:	10.1055/s-2004-832615