Interaction between GLP-1 and CCK-33 in inhibiting food intake and appetite in men

1 Clinical Research Center and Department of Research and 2 Division of Gastroenterology, University Hospital, CH-4031 Basel, Switzerland Submitted 15 October 2003 ; accepted in final form 14 April 2004 Glucagon-like peptide-1 (GLP-1) and CCK-33 were intravenously infused alone or in combination into normal weight men for 60 min before they were served a lunch of ham sandwiches, chocolate mousse, and orange juice. Infusion of GLP-1 (dose: 0.9 pmol·kg –1 ·min –1 ) or CCK-33 (dose: 0.2 pmol·kg –1 ·min –1 ) each reduced calorie intake of the test meal. However, simultaneous infusion of these peptide doses reduced calorie intake less than the sum of the peptides' individual effects. Infusions of the same doses of GLP-1 plus CCK-33 had neither individual nor interactive effects on meal size or calorie consumption. The combination of GLP-1 plus CCK-33 induced, however, a significant reduction in hunger feelings in the premeal period ( P = 0.036 vs. all other treatments). In summary, intravenous infusion of near physiological doses of CCK-33 and GLP-1 produced specific inhibitions of hunger feeling in men; the simultaneous infusion resulted in an infra-additive reduction in calorie consumption, rejecting thereby the hypothesis that the two peptides exert a positive synergistic effect on food intake compared with the effects observed with infusion of individual peptides. In conclusion, CCK and GLP-1 are meal-related satiety signals that are released from the gastrointestinal tract during food intake. glucagon-like peptide; cholecystokinin Address for reprint requests and other correspondence: C. Beglinger, Division of Gastroenterology, Univ. Hospital, CH-4031 Basel, Switzerland (E-mail: beglinger{at}tmr.ch )