Proteomic signatures for daunomycin and adriamycin in Bacillus subtilis

The influence of the two anthracyclin antibiotics daunomycin and adriamycin on the proteome of Bacillus subtilis was investigated. They intercalate in the double helix causing strand breaks. Both compounds induce proteins related to DNA damage and oxidative stress as indicated by the induction of some members of the PerR and the DinR-regulon. The mild induction of some members of the HrcA- and the CtsR-regulon may indicate protein oxidation as well. Furthermore, an influence on the σB-dependent general stress response was shown. These data show that the proteomic signature is a valuable experimental tool for a comprehensive evaluation of the action of different drugs.