Sodium pyruvate protects against H(2)O(2) mediated apoptosis in human neuroblastoma cell line-SK-N-MC

Free radicals are involved in neuronal damage. The present study was aimed to investigate the protective effect of sodium pyruvate-a free radical scavenger against hydrogen peroxide (H(2)O(2)) induced apoptosis in human neuroblastoma cell line-SK-N-MC. On exposure to H(2)O(2) (0.025 mM) cells exhibi...

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Veröffentlicht in:	Journal of chemical neuroanatomy 2003-10, Vol.26 (2), p.109
Hauptverfasser:	Jagtap, Jayashree C, Chandele, Anmol, Chopde, B A, Shastry, Padma
Format:	Artikel
Sprache:	eng
Schlagworte:	Antioxidants Apoptosis - drug effects Blotting, Western Brain Neoplasms - pathology Caspase 3 Caspases - biosynthesis Cell Line, Tumor Cell Survival Colorimetry DNA Fragmentation Electrophoresis, Polyacrylamide Gel Flow Cytometry Humans Hydrogen Peroxide - antagonists & inhibitors Hydrogen Peroxide - toxicity In Situ Nick-End Labeling Membrane Potentials - physiology Neuroblastoma - pathology Neuroprotective Agents Oxidants - toxicity Pyruvic Acid - pharmacology
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Zusammenfassung:	Free radicals are involved in neuronal damage. The present study was aimed to investigate the protective effect of sodium pyruvate-a free radical scavenger against hydrogen peroxide (H(2)O(2)) induced apoptosis in human neuroblastoma cell line-SK-N-MC. On exposure to H(2)O(2) (0.025 mM) cells exhibited apoptosis within 24 h, demonstrating a high caspase 3 activity by 3 h followed by cleavage of PARP that was maximum at 24 h. A break down in the mitochondrial membrane potential was observed 3 h onwards. Sodium pyruvate protected cells significantly (P
ISSN:	0891-0618