Exploratory and confirmatory gene expression profiling of mac1Delta

Exploratory outlier identification methods and confirmatory gene expression studies showed induction of the iron regulon in Saccharomyces cerevisiae lacking Mac1p, a copper-responsive transcription factor. The Aft1p/Aft2p binding motif was the most discriminating motif between up- and down-regulated...

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Veröffentlicht in:	The Journal of biological chemistry 2004-02, Vol.279 (6), p.4450
Hauptverfasser:	De Freitas, Jeane Maria, Kim, Jeung Hyoun, Poynton, Helen, Su, Trent, Wintz, Henri, Fox, Tama, Holman, Patty, Loguinov, Alex, Keles, Sunduz, van der Laan, Mark, Vulpe, Chris
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Copper - metabolism DNA, Fungal - genetics Gene Deletion Gene Expression Profiling Genes, Fungal Iron - metabolism Models, Biological Mutation Nuclear Proteins - genetics Nuclear Proteins - metabolism Oligonucleotide Array Sequence Analysis Phenotype Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Transcription Factors - genetics Transcription Factors - metabolism
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Zusammenfassung:	Exploratory outlier identification methods and confirmatory gene expression studies showed induction of the iron regulon in Saccharomyces cerevisiae lacking Mac1p, a copper-responsive transcription factor. The Aft1p/Aft2p binding motif was the most discriminating motif between up- and down-regulated genes, and we identified new genes potentially regulated by Aft1p/Aft2p. In addition, multiple genes encoding proteins containing Fe-S clusters were down-regulated suggesting metabolic reorganization to conserve iron in mac1Delta. Null mutants of each of the differentially expressed genes were characterized for copper- or iron-related phenotypes. New or additional support for a role in copper and iron homeostasis is provided in this study for the gene products of AKR1, MRS4, PCA1, SSU1, TIS11, YBR047W, YHL035C, YHR045W, YLR047C, YLR126C, and YTP1.
ISSN:	0021-9258