The multiplication of herpes simplex virus: II. The relation between protein synthesis and the duplication of viral DNA in infected HEp-2 cells
The uptake of thymidine-H 3 into the ethanol-insoluble fraction (DNA) of herpes simplex virus-infected HEp-2 cells is blocked by puromycin added to the medium prior to 4 hours after infection. After 4 hours of infection, the uptake of thymidine continues at a reduced rate dependent on the time of ad...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1964-02, Vol.22 (2), p.262-269 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Roizman, Bernard Roane, Philip R. |
| description | The uptake of thymidine-H
3 into the ethanol-insoluble fraction (DNA) of herpes simplex virus-infected HEp-2 cells is blocked by puromycin added to the medium prior to 4 hours after infection. After 4 hours of infection, the uptake of thymidine continues at a reduced rate dependent on the time of addition of the drug. On the basis of the apparent buoyant density on equilibrium sedimentation in CsCl it was determined that both viral and cellular DNA are made in infected cells treated with puromycin 6 hours after infection.
The equilibrium sedimentation studies also revealed that cellular DNA is made between 0 and 3 hours after infection with herpes simplex virus. Subsequently, both viral and cellular DNA are made, but not at the same rates. |
| doi_str_mv | 10.1016/0042-6822(64)90011-X |
| format | Article |
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3 into the ethanol-insoluble fraction (DNA) of herpes simplex virus-infected HEp-2 cells is blocked by puromycin added to the medium prior to 4 hours after infection. After 4 hours of infection, the uptake of thymidine continues at a reduced rate dependent on the time of addition of the drug. On the basis of the apparent buoyant density on equilibrium sedimentation in CsCl it was determined that both viral and cellular DNA are made in infected cells treated with puromycin 6 hours after infection.
The equilibrium sedimentation studies also revealed that cellular DNA is made between 0 and 3 hours after infection with herpes simplex virus. Subsequently, both viral and cellular DNA are made, but not at the same rates.</description><subject>Anti-Bacterial Agents</subject><subject>Antimetabolites</subject><subject>DNA</subject><subject>DNA, Viral</subject><subject>Metabolism</subject><subject>Protein Biosynthesis</subject><subject>Proteins - metabolism</subject><subject>Puromycin</subject><subject>Simplexvirus</subject><subject>Thymidine</subject><subject>Tritium</subject><subject>Viral Proteins</subject><subject>Virus Cultivation</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1964</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUFLw0AUhBdRbK3-A5E96iH17W6ySTwIpVZbKHqp0NuSbN7SlSQN2aTaX-FfNrEqeHrz4GMYZgi5ZDBmwOQtgM89GXF-Lf2bGIAxb31Ehgxi6YHw2TEZ_iEDcubcG3R_GMIpGTCfyUAINiSfqw3Sos0bW-VWJ43dlnRr6AbrCh11tqhy_KA7W7fuji4WY9rzNeYHMsXmHbGkVb1t0JbU7ctmg846mpQZ7STN2n--nVGS04fnCe1oWxrUDWZ0Pqs8TjXmuTsnJybJHV783BF5fZytpnNv-fK0mE6WHrIwbLwIDNcgDIAUQcDSNE5lYALJAzSYxAaEQD8NmDAcNUKgY2RpbJBxbUwU-WJErg6-VZsWmKmqtkVS79VvMx1wfwCwS7GzWCunLZYaM1t3qVW2tYqB6qdQfc-q71lJX31PodbiC4qpe_A</recordid><startdate>196402</startdate><enddate>196402</enddate><creator>Roizman, Bernard</creator><creator>Roane, Philip R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>196402</creationdate><title>The multiplication of herpes simplex virus: II. The relation between protein synthesis and the duplication of viral DNA in infected HEp-2 cells</title><author>Roizman, Bernard ; Roane, Philip R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e177t-80f2c03f0063551bb9b65f5625efea9f033e4b513f2ece05c9e1b9fe12cff8843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1964</creationdate><topic>Anti-Bacterial Agents</topic><topic>Antimetabolites</topic><topic>DNA</topic><topic>DNA, Viral</topic><topic>Metabolism</topic><topic>Protein Biosynthesis</topic><topic>Proteins - metabolism</topic><topic>Puromycin</topic><topic>Simplexvirus</topic><topic>Thymidine</topic><topic>Tritium</topic><topic>Viral Proteins</topic><topic>Virus Cultivation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roizman, Bernard</creatorcontrib><creatorcontrib>Roane, Philip R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roizman, Bernard</au><au>Roane, Philip R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The multiplication of herpes simplex virus: II. The relation between protein synthesis and the duplication of viral DNA in infected HEp-2 cells</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1964-02</date><risdate>1964</risdate><volume>22</volume><issue>2</issue><spage>262</spage><epage>269</epage><pages>262-269</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>The uptake of thymidine-H
3 into the ethanol-insoluble fraction (DNA) of herpes simplex virus-infected HEp-2 cells is blocked by puromycin added to the medium prior to 4 hours after infection. After 4 hours of infection, the uptake of thymidine continues at a reduced rate dependent on the time of addition of the drug. On the basis of the apparent buoyant density on equilibrium sedimentation in CsCl it was determined that both viral and cellular DNA are made in infected cells treated with puromycin 6 hours after infection.
The equilibrium sedimentation studies also revealed that cellular DNA is made between 0 and 3 hours after infection with herpes simplex virus. Subsequently, both viral and cellular DNA are made, but not at the same rates.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>14165331</pmid><doi>10.1016/0042-6822(64)90011-X</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Virology (New York, N.Y.), 1964-02, Vol.22 (2), p.262-269 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_pubmed_primary_14165331 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Free E-Journal (出版社公開部分のみ） |
| subjects | Anti-Bacterial Agents Antimetabolites DNA DNA, Viral Metabolism Protein Biosynthesis Proteins - metabolism Puromycin Simplexvirus Thymidine Tritium Viral Proteins Virus Cultivation |
| title | The multiplication of herpes simplex virus: II. The relation between protein synthesis and the duplication of viral DNA in infected HEp-2 cells |
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