The multiplication of herpes simplex virus: II. The relation between protein synthesis and the duplication of viral DNA in infected HEp-2 cells

The uptake of thymidine-H 3 into the ethanol-insoluble fraction (DNA) of herpes simplex virus-infected HEp-2 cells is blocked by puromycin added to the medium prior to 4 hours after infection. After 4 hours of infection, the uptake of thymidine continues at a reduced rate dependent on the time of addition of the drug. On the basis of the apparent buoyant density on equilibrium sedimentation in CsCl it was determined that both viral and cellular DNA are made in infected cells treated with puromycin 6 hours after infection. The equilibrium sedimentation studies also revealed that cellular DNA is made between 0 and 3 hours after infection with herpes simplex virus. Subsequently, both viral and cellular DNA are made, but not at the same rates.