Increased expression of endothelin-1 and its mitogenic receptor ETA in human papillary thyroid carcinoma

Summary objective Since the isolation of endothelin‐1 (ET‐1) in 1988, there has been tremendous interest in the pathophysiological roles of ET‐1 as a vasoconstrictive and mitogenic peptide. Whereas ET‐1 is mainly released by vascular endothelial cells, it also proved to be produced by various tissues including the thyroid. Because of its mitogenic properties in malignancy and its role as an inflammatory modulator, ET‐1 could be involved in thyroid carcinogenesis and thyroiditis. design and patients Studies were performed in human thyroid samples obtained at the time of surgery from 39 men and women aged 15–72 years. Thyroid samples were classified in four groups according to conventional histology: normal thyroid (n = 7) papillary thyroid carcinoma (n = 12), Hashimoto's thyroiditis (n = 9) and benign nontoxic nodular goitres (n = 11). Immunohistochemistry and real‐time quantitative polymerase chain reaction were used to determine the expression of ET‐1 and its receptors (ETAR and ETBR). results ET‐1 and ETAR mRNA levels were, respectively, 3·8 ± 1·3 and 4·1 ± 1·5 times greater (P