Activation of caspase 9 in a rat model of experimental glaucoma

Activation of caspase 9 in a rat model of experimental glaucoma

Purpose. We investigated retinal ganglion cell (RGC) death and activation of caspase 9 in rats with experimental glaucoma. Methods. Elevated intraocular pressure (IOP) was induced in rats using the Morrison model. Surviving backlabeled RGC were counted and TUNEL staining detected apoptosis. Procaspa...

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Veröffentlicht in:Current eye research 2002-12, Vol.25 (6), p.389-395
Hauptverfasser: Hänninen, Virve A., Pantcheva, Mina B., Freeman, Ellen E., Poulin, Nathaniel R., Grosskreutz, Cynthia L.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Apoptosis
Caspase 9
Caspases - chemistry
Caspases - metabolism
Cell Count
Enzyme Activation
Enzyme Precursors - metabolism
Glaucoma - enzymology
Glaucoma - pathology
Glaucoma - physiopathology
Immunoblotting
Immunohistochemistry - methods
In Situ Nick-End Labeling
Intraocular Pressure
Male
Rats
Rats, Inbred BN
Retinal Ganglion Cells - enzymology
Staining and Labeling
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Zusammenfassung:Purpose. We investigated retinal ganglion cell (RGC) death and activation of caspase 9 in rats with experimental glaucoma. Methods. Elevated intraocular pressure (IOP) was induced in rats using the Morrison model. Surviving backlabeled RGC were counted and TUNEL staining detected apoptosis. Procaspase 9 expression and activated caspase 9 were studied by immunoblot and immunohistochemistry. Results. IOP correlated with surviving RGC. TUNELpositive RGC were observed in animals with elevated IOP. Procaspase 9 levels increased with IOP intensity. Cleaved caspase 9 was detected by immunoblot only in rats with peak IOP above 35mmHg for =6 days. Cleaved caspase 9 staining was seen only in the ganglion cell layer of retinas from rats with peak IOP =32mmHg. Conclusions. RGC loss is correlated with IOP in experimental glaucoma. These results support activation of caspase 9, the intrinsic caspase cascade, in RGC death in experimental glaucoma.
ISSN:0271-3683
1460-2202
DOI:10.1076/ceyr.25.6.389.14233