Construction of a vector plasmid for use in Gluconobacter oxydans

A host vector system in Gluconobacter oxydans was constructed. An Acetobacter-Escherichia coli shuttle vector was introduced with the efficiency of 10 4 transformants/μg of DNA. Next, aiming for a self-cloning vector, we found a cryptic plasmid (which we named pAG5) of 5648 bp in G. oxydans strain I...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-01, Vol.67 (1), p.211-213
Hauptverfasser:	Tonouchi, N. (Ajinomoto Co. Inc., Kawasaki, Kanagawa (Japan). Central Research Labs.), Sugiyama, M, Yokozeki, K
Format:	Artikel
Sprache:	eng
Schlagworte:	BACTERIA Biological and medical sciences cryptic plasmid Escherichia coli - genetics Fundamental and applied biological sciences. Psychology GENETIC VECTORS Gluconobacter Gluconobacter oxydans - genetics host-vector system Molecular Sequence Data Open Reading Frames - genetics PLASMIDS Plasmids - genetics Restriction Mapping
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Beschreibung
Zusammenfassung:	A host vector system in Gluconobacter oxydans was constructed. An Acetobacter-Escherichia coli shuttle vector was introduced with the efficiency of 10 4 transformants/μg of DNA. Next, aiming for a self-cloning vector, we found a cryptic plasmid (which we named pAG5) of 5648 bp in G. oxydans strain IFO 3171, and sequenced the nucleotides. The plasmid seemed to have only one open reading flame (ORF) for a possible replication protein. Shuttle vectors of Gluconobacter-E. coli were constructed with the plasmid pAG5 and an E. coli vector, pUC18.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.67.211