Synergism between neutron radiation and diethylstilbestrol in the production of mammary adenocarcinomas in the rat

When young female A X C rats were given 9.6 rads of 0.43-MeV neutrons, 32 of 33 survived a 50-week follow-up period, 2 rats developed a total of 3 mammary adenocarcinomas, and 3 rats developed a total of 4 mammary fibroadenomas. For 25 rats implanted with a 20-mg pellet containing 5 mg diethylstilbestrol and 15 mg cholesterol, average survival was 284 days; 22 rats developed a total of 182 mammary adenocarcinomas, and 21 rats developed a pituitary tumor. When diethylstilbestrol was given 2 days before neutron radiation to 35 rats, the average survival was 239 days; 32 rats developed a total of 842 mammary adenocarcinomas, 1 rat developed a single mammary fibroadenoma, and 34 rats developed a pituitary tumor. All of the 31 control rats survived the 50-week study period, and none developed tumors. Twenty-one of the rats that received both diethylstilbestrol and neutron radiation and 1 rat that received only diethylstilbestrol exhibited a multiple mammary adenocarcinoma response with a range of 18 to 72 mammary adenocarcinomas per rat. These results were interpreted to mean that a synergistic interaction between diethylstilbestrol and neutron radiation on mammary adenocarcinoma formation occurs in terms of an earlier onset and a larger number of mammary adenocarcinomas. These results confirm and complement a previously reported synergistic interaction between diethylstilbestrol and X-radiation on mammary adenocarcinoma formation in A X C female rats.